[Histonet] formaldehyd concentration and IHC

[Bryan Hewlett]

Gudrun,

A minimum fixation time of 24 hours, in the commonly used concentration of
4% w/v formaldehyde in phosphate buffer pH 7.2-7.4, has worked well in my
hands for over 40 years of IHC, using hundreds of different antibodies.

Bryan


----- Original Message -----
From: "Gudrun Lang" <gu.lang <@t> gmx.at>
To: "Histonetliste (Histonetliste)" <histonet <@t> lists.utsouthwestern.edu>
Sent: Thursday, February 17, 2005 3:12 PM
Subject: [Histonet] formaldehyd concentration and IHC


> In addition to the fixation-time-discussion a question about the
> concentration. What formaldehyd concentration do you prefer to get the
> optimal IHC- result?
>
> 4% or higher?; or does it really matter?
>
>
>
> Gudrun Lang
>
>
>
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