[Histonet] antigen retrieval question

[Jim Staruk]

If I remember correctly, mine was an anti-collagen I or II that did not work
after sitting in buffer overnight.  I also know that one of my part-timers
tried this at the hospital he works at and they also had disastrous results
on day #2.

______________________
    Jim Staruk
Mass Histology Service
www.masshistology.com

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Kim Merriam
Sent: Friday, February 18, 2005 7:42 AM
To: Jim Staruk; 'Patsy Ruegg'; ihcrg <@t> neo.agsci.colostate.edu
Cc: histonet <@t> pathology.swmed.edu
Subject: RE: [Histonet] antigen retrieval question

Patsy,
 
I have done this many times before, and have never had any issues with
re-masking.  Why would the sites be re-masked if they are not subjected to
formalin again?  If I am staining a big batch of slides (anything over 100
slides at a time), I do the depar and retrieval on the first day and all of
the antibody stuff on the second day.  Maybe it just depends on the
protein/antibody?
 
This has peaked my interest.  I am curious to see if anyone else has
encountered this problem.
 
Kim Merriam
Novartis
Cambridge, MA