[Histonet] mouse skin biopsies

[Molinari, Betsy]

Sharon,
I have done a few, they were for monitoring the hair growth, so I had
only a small tag of skin. They were fixed in 10% NBF and I ran them in a
same day run. Seemed to work fine for us.
70% 30min
80% 30min		
95% 30min
95% 30min	
100% 30min
100% 30min
xylene 45min
xylene 45min
paraffin 30min
paraffin 30min
paraffin 30min	
paraffin 30min
 Betsy Molinari HT(ASCP)
Texas Heart Institute
Cardiovascular Pathology
6770 Bertner Ave
Houston,TX 77090
832-355-6524
832-355-6812 (fax)

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Sharon
Cooperman
Sent: Wednesday, February 16, 2005 8:44 PM
To: Histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] mouse skin biopsies

Dear Histonetters,

I need to do a skin biopsy on a mouse.  I've never done any skin 
stuff before.  Could someone share a protocol with me on how to do 
this?  (even info on human skin biopsies would be helpful).  Are skin 
biopsies usually formalin fixed and paraffin embedded?  If anyone has 
a protocol or a reference I could look up it would be greatly 
appreciated.

Ignorantly yours,
Sharon
-- 
Sharon Cooperman        	     <scoop <@t> mail.nih.gov>
NIH, NICHD, CBMB                     301.435-8417
Building 18T, room 101               301.402-0078 fax
Bethesda, MD 20892

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