[Histonet] Question on blocking serum

[Tony Henwood]

Jim,

I would use either the same sera that the primary was raised in or whatever
sera from another species that I had on hand. I hope that any non-specific
binding of the primary AB would be prevented by this blocking sera
incubation.
Regards

Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager & Senior Scientist
The Children's Hospital at Westmead,
Locked Bag 4001, Westmead, 2145, AUSTRALIA.
Tel: 612 9845 3306
Fax: 612 9845 3318






-----Original Message-----
From: Jim Staruk [mailto:jstaruk <@t> masshistology.com] 
Sent: Thursday, 17 February 2005 8:35 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Question on blocking serum


All,

I always block non-specific antibodies with a 20% solution of normal serum
of the same species the secondary antibody was produced in prior to the
initial primary antibody step.  What would I use as a blocking solution if
the primary antibody is already biotin labeled? A 20% solution of normal
serum of the same species the primary antibody was raised in?

Anyone planning on invoicing me for their answer need not respond to this
question.

Thanks in advance

Jim

______________________
    Jim Staruk
Mass Histology Service
www.masshistology.com




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