[Histonet] Question on blocking serum

[Jim Staruk]

All,

I always block non-specific antibodies with a 20% solution of normal serum
of the same species the secondary antibody was produced in prior to the
initial primary antibody step.  What would I use as a blocking solution if
the primary antibody is already biotin labeled? A 20% solution of normal
serum of the same species the primary antibody was raised in?

Anyone planning on invoicing me for their answer need not respond to this
question.

Thanks in advance

Jim

______________________
    Jim Staruk
Mass Histology Service
www.masshistology.com