[Histonet] Antibodies to Thy1.1 in FFPE mouse tissue

[Randolph-Habecker, Julie]

Hello all,
I am working with an investigator who would like to detect Thy1.1 (CD90.1)
positive cells which were infused into a mouse expressing Thy1.2 (CD90.2).
They are doing this retrospectively and already have FFPE tissue (so I can't
use frozen tissue). I tried the OX-7 clone from BD with no antigen
retrieval, pH 6 tris/steam, EDTA/steam, and proteinase K using the ARK kit
for detection on spleens from Thy1.1 mice..... But I didn't get any signal.

Has anyone had good results with a Thy1.1 antibody on FFPE mouse tissue?
Please let me know!

Thanks,

Julie

Julie Randolph-Habecker, Ph.D.
Experimental Histopathology Shared Resources
Fred Hutchinson Cancer Research Center
1100 Fairview Ave. N, G1-300
PO Box 19023
Seattle, WA 98109-1024
Tel: (206) 288-1187
FAX: (206) 288-1345
jhabecke <@t> fhcrc.org <mailto:jhabecke <@t> fhcrc.org>



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