[Histonet] Water bath temperature[Scanned]

[Kemlo Rogerson]

I'm not too proud to admit that you have me on that. Can't win all the time,
I apologise.

Kemlo Rogerson
Cellular Pathology Manager
East Lancashire Hospitals NHS Trust
DD. 01254-294162
Mobile 0774-9754194
 

-----Original Message-----
From: Marshall Terry Dr, Consultant Histopathologist
[mailto:Terry.Marshall <@t> rothgen.nhs.uk] 
Sent: 15 February 2005 13:37
To: Kemlo Rogerson; Rebecca Barnhart; histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] Water bath temperature[Scanned]

Thank you Kemlo for your insightful comments.

However, though necessarily amateurish about things technical, I can't help
feeling that:-

"I tried the only other thing left to change, I
lowered the temperature of the water bath from 45 to 38 and this worked.", 

means the exact opposite of what you said, viz.

"Because, if you read the thread properly, it doesn't affect sibling H&E's
cut from the same block but floated out on a hotter water bath."

Perhaps, if you read the thread properly, you may prevent making a dick of
yourself with these gratuitously rude comments.

Furthermore, until the cause of pink disease is known, your arguement that
it couldn't have been that merely because schedule A prevented it and
schedule B didn't (however befuddled you were about which was which) does
not follow.


Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path
 Consultant Pathologist
 Rotherham General Hospital
 South Yorkshire
 England
        terry.marshall <@t> rothgen.nhs.uk

From: Kemlo Rogerson

Because, if you read the thread properly, it doesn't affect sibling H&E's
cut from the same block but floated out on a hotter water bath. Maybe
technical ought to be left to the 'technicians'. IMHO.

-----Original Message-----
From: Marshall Terry Dr,Consultant Histopathologist

Why from the description is it not just our old friend pink disease?
(BayleyJH {1949}J.Path.BAct., 61,448)
Are the polka dots too small or too many or too invariable, or any
combination thereof?
As usual, a picture would be worth a thousand words.

She wore an itsy bitsy teeny weeny polka dot bikini .......
I was never sure if it was the polka dots that were teeny weeny. 
Probably not.

Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path
 Consultant Pathologist
 Rotherham General Hospital
 South Yorkshire
 England
        terry.marshall <@t> rothgen.nhs.uk

-----Original Message-----
From: Rebecca Barnhart [mailto:RBARNHART <@t> summithealth.org]
Sent: 11 February 2005 19:24
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Water bath temperature


We were having a problem that the tissue would not take up the
hematoxlyin but would take up the eosin but only in some areas, so it
looked like polka dots.  We started one by one changing solution trying
to figure out where it was coming from.  We had all fresh solutions and
still had polka dots.  I tried the only other thing left to change, I
lowered the temperature of the water bath from 45 to 38 and this worked.
 We currently are using ParaPlast Xtra with a melting point of 52.  We
have all tried figure out why the temperature in the water bath will
make the difference.  Several times I have cut the same tissue at
different temperatures ranging from 38 - 45 and every time there are
polka dots at the higher temperatures.  Does anyone have any idea why
this would be?  

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