[Histonet] Microglia

[Geoff McAuliffe]

Hi Fred:

   For mouse, Mac1 or F4/80 work well on frozen sections of lightly 
fixed (buffered formalin) material. For rat, OX-42 is the equivalent of 
Mac1. ED-1 is another marker for rat microglia. I don't have experience 
with human tissues. While it is possible to get some of these markers to 
work on paraffin embedded material, frozen sections of fixed material 
are a better choice.

Geoff

Fred Ross-Cisneros wrote:

>Dear All,
> 
>What would be a specific antibody (that's commercially availble) to CNS
>micoglia? I've heard that CD-68 and ED-1 can be used. From your
>experience, what is the best for human and/or murine/rodent tissues.
> 
>Thanks.
> 
>Fred
>  
> 
>
>Fred N. Ross-Cisneros
>
>Sadun Neuro-Ophthalmology Lab
>
>USC Keck School of Medicine and
>
>Doheny Eye Institute
>
>1355 San Pablo Street, DVRC 311
>
>Los Angeles, CA 90033-1026
>
>Tel.: (323) 442-6667
>
>Fax: (323) 442-6688
>
> 
>
> 
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Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583; fax: -4029 
mcauliff <@t> umdnj.edu
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