[Histonet] RE: sorted cells from spleen to stain

Osborn, Sharon sharon.osborn <@t> dnax.org
Thu Feb 10 13:51:04 CST 2005

Histoland experts.....
	I have a researcher who is macerating spleen, sorting the cells,
lysing out the red cells and wants a good stain to identify the cells,
primarily B cells with some T cells. These are on cytospin samples.  He has
used T-Blue, Wright-Giemsa and just Giemsa.  It seems the staining could be
better for it is not showing nuclear detail that the PI thinks it should be.
Does anyone have suggestions for a different histochemical technic or ways
to tweak what he is currently doing?  Thanks.

Sharon Osborn
Palo Alto, CA

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