[Histonet] RE: sorted cells from spleen to stain

[Osborn, Sharon]

Histoland experts.....
	I have a researcher who is macerating spleen, sorting the cells,
lysing out the red cells and wants a good stain to identify the cells,
primarily B cells with some T cells. These are on cytospin samples.  He has
used T-Blue, Wright-Giemsa and just Giemsa.  It seems the staining could be
better for it is not showing nuclear detail that the PI thinks it should be.
Does anyone have suggestions for a different histochemical technic or ways
to tweak what he is currently doing?  Thanks.

Sharon Osborn
DNAX 
Palo Alto, CA


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