[Histonet] Decloaking chamber problem
MAUGER <@t> email.chop.edu
Wed Feb 9 13:36:31 CST 2005
We use the larger, rectangular 'boats' that hold a plastic slide
rack(24 slide capacity) We have never had boiling over. More retrieval
soln is used, but it saves you from repeating,and is more standardized.
>>> "Brianna Jackson" <bjackson <@t> unipathllc.com> 02/09/05 12:39 PM >>>
This question is for those of you using the Biocare Medical decloaking
chamber or the Dako Pascal decloaking chamber. We're having trouble
the coplin jars boiling over during retrieval during the
portion of retrieval. After the alarm sounds we turn the decloaker off
don't disturb the chamber until it has completely depressurized.
started using the taller coplin jars from Scienceware and it still
It doesn't seem to be dependant on the type of retrieval solution, the
amount of retrieval solution in the coplin jar, barometric pressure,
number of slides or whether or not we use the little perforated disk
shield) in the bottom of the decloaker. Our patient specimens are
OK, being at the bottom of the slide, but we have to repeat cases that
boiled over because the control tissue at the top of the slide no
stains by IHC.
If anyone has figured out a solution to this problem any
would be appreciated.
Brianna Jackson, BS, HTL(ASCP), QIHC
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