[Histonet] Decloaking chamber problem

Jan Shivers shive003 <@t> umn.edu
Wed Feb 9 12:04:04 CST 2005

I use Tissue Tek rectangular plastic containers and slide racks.  No problem
with overheating/overboiling, and I decloak in Dako Target Retrieval
Solution for 20 minutes, followed by a 25 minute cooldown inside the cooker.

Jan Shivers
U of MN Vet Diag Lab

----- Original Message ----- 
From: "Brianna Jackson" <bjackson <@t> unipathllc.com>
To: <histonet <@t> lists.utsouthwestern.edu>
Sent: Wednesday, February 09, 2005 11:39 AM
Subject: [Histonet] Decloaking chamber problem

> Hello Everyone,
> This question is for those of you using the Biocare Medical decloaking
> chamber or the Dako Pascal decloaking chamber.  We're having trouble with
> the coplin jars boiling over during retrieval during the depressurizing
> portion of retrieval.  After the alarm sounds we turn the decloaker off
> don't disturb the chamber until it has completely depressurized.  We've
> started using the taller coplin jars from Scienceware and it still
> It doesn't seem to be dependant on the type of retrieval solution, the
> amount of retrieval solution in the coplin jar, barometric pressure, the
> number of slides or whether or not we use the little perforated disk (heat
> shield) in the bottom of the decloaker.  Our patient specimens are always
> OK, being at the bottom of the slide, but we have to repeat cases that
> boiled over because the control tissue at the top of the slide no longer
> stains by IHC.
>             If anyone has figured out a solution to this problem any help
> would be appreciated.
> Thanks,
> Brianna Jackson, BS, HTL(ASCP), QIHC
> UniPath
> Denver, CO
> 303-512-2220
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