[Histonet] Rubber cement (for in situ)

[Patsy Ruegg]

I have used just a glass coverslip laying on over the hybridizing reagent
without sealing it at all, then the glass can be floated off in buffer.  I
hope I am understanding this inquiry.
Patsy 

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Elizabeth
Chlipala
Sent: Thursday, February 03, 2005 10:52 AM
To: '- -'; histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] Rubber cement (for in situ)

We use Frame-Seal from MJ Research, Inc. www.mjr.com (888)735-8437

Liz

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC
P.O. Box 18592 Boulder, Colorado 80308
Office: (303) 735-5001
Fax: (303) 735-3540
liz <@t> premierlab.com
www.premierlab.com
 
Ship to Address:
Premier Laboratory
University of Colorado
MCDB, Room A3B40
Boulder, Colorado 80309

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of - -
Sent: Thursday, February 03, 2005 10:21 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Rubber cement (for in situ)

Hello,
 
I am starting to work up a protocol for in situ hybridization and discovered
I need to seal my slides because of the high temps and long incubation.  Can
anyone help me with the following questions:  
 
a. I noticed the rubber cement is quite fluid.  Using the brush that it
comes with the can (or another method), how does one go about applying it
after your sample is coverslipped?
 
b. I've heard about fumes ... what concerns should I have; safety
precautions should I take? (my normal range of temperature will be between
43 and 55 degrees Celcius within a Boekel Slide Moat or all purpose
incubator)
 
c. Are there better methods to use when sealing slides? 
 
d. What is the best way to remove the rubber cement/coverslip after
performing hybridization?
 
THANK YOU ALL VERY MUCH!!!!!

		
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