[Histonet] Rubber cement (for in situ)

Connolly, Brett M brett_connolly <@t> merck.com
Thu Feb 3 11:46:24 CST 2005

Don't use the brush. Use 22 mm wide coverslips instead of 24 mm. With a
plastic transfer pipet lay a thin line of rubber cement at the junction of
the coverslip and the slide. After hybridization the solidified rubber
cement easily peels away. Actually, your temps are relatively low for ISH,
we've hybridized at >65C. 


Brett M. Connolly, Ph.D.
Merck & Co., Inc.
MRL, Imaging Research
PO Box 4
West Point, PA 19486
PH 215-652-2501
fax. 215-652-2075
e-mail. brett_connolly <@t> merck.com

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of - -
Sent: Thursday, February 03, 2005 12:21 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Rubber cement (for in situ)

I am starting to work up a protocol for in situ hybridization and discovered
I need to seal my slides because of the high temps and long incubation.  Can
anyone help me with the following questions:  
a. I noticed the rubber cement is quite fluid.  Using the brush that it
comes with the can (or another method), how does one go about applying it
after your sample is coverslipped?
b. I've heard about fumes ... what concerns should I have; safety
precautions should I take? (my normal range of temperature will be between
43 and 55 degrees Celcius within a Boekel Slide Moat or all purpose
c. Are there better methods to use when sealing slides? 
d. What is the best way to remove the rubber cement/coverslip after
performing hybridization?

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