[Histonet] CD-20 nuclear staining....

[Bonnie Whitaker]

If the pH of the HIER solution is off (too acid) you can see this artifact,
as well.  

Bonnie Whitaker


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Patsy Ruegg
Sent: Wednesday, February 02, 2005 2:25 PM
To: 'Richard Cartun'; Histonet <@t> lists.utsouthwestern.edu;
cuttingedgehistology <@t> yahoo.com
Subject: RE: [Histonet] CD-20 nuclear staining....


Over heating during hier can cause false nuclear staining? Patsy 

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Richard
Cartun
Sent: Wednesday, February 02, 2005 8:23 AM
To: Histonet <@t> lists.utsouthwestern.edu; cuttingedgehistology <@t> yahoo.com
Subject: Re: [Histonet] CD-20 nuclear staining....

I have never seen nuclear staining with CD20 (clone L26).  Which
pretreatment and detection system are you using?

Richard

Richard W. Cartun, Ph.D.
Director, Immunopathology & Histology
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 545-1596
(860) 545-0174 Fax

>>> Brandon Stokes <cuttingedgehistology <@t> yahoo.com> 02/01/05 09:26PM
>>>
Hello fellow histo-netters,
 
I was wondering if anyone else has ever experienced nuclear staining
occuring when using the CD-20 antibody.  The tonsil control will look
great....then you look at the patient tissue(almost always skin for us) and
some of the nuclei of the keratinocytes in the epidermis and even some
nuclei in the dermis might light up bright red(we use AEC).  You can
actually see the nuclear detail very well.  This problem doesn't happen all
the time so I am not sure what it could be.  
 
Any advice would be greatly appreciated.
 
 
Brandon Stokes, HT(ASCP)
Lab Manager
Cutting Edge Histology Services, LLC
 

		
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