[Histonet] Alcian Blue problem

[Philip Oshel]

Do you filter your stain?
Phil

>I also have occasional problems with the 2.5 Alcian Blue.  Greenish
>parcipitants on top of the slide.  Same solutions...no consistency of
>trouble...
>
>>>>  Tom McNemar <TMcNemar <@t> lmhealth.org> 2/2/2005 4:55:07 AM >>>
>We have started to see a hazy/cloudiness to our alcian blue PAS slides.
>  We
>don't see this on any other slide/stain.  We use the same slides,
>reagents,
>and procedure that we have always used.  The stain works fine and the
>haze
>is on the entire slide, not just the tissue.  We routinely use the
>Fisherbrand red control slides and have tried other slides and still
>have
>the same problem.  I use the Poly Scientific reagents (alcian blue ph
>2.5)
>and have switched them all out for new  reagents but still have the
>haze.
>We have switched out, altered, and tried everything I can think of.  I
>am
>totally baffled.  Hope you folks have some ideas.
>
>Tom Mc Nemar HT(ASCP)
>Histology Supervisor
>Licking Memorial Hospital
>Newark, Ohio 43055
>
>
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-- 
Philip Oshel
Supervisor, BBPIC microscopy facility
Department of Animal Sciences
University of Wisconsin
1675 Observatory Drive
Madison,  WI  53706
voice: (608) 263-4162
fax: (608) 262-5157 (dept. fax)