[Histonet] Alcian Blue problem

[Kathleen Boozer]

I also have occasional problems with the 2.5 Alcian Blue.  Greenish
parcipitants on top of the slide.  Same solutions...no consistency of
trouble...

>>> Tom McNemar <TMcNemar <@t> lmhealth.org> 2/2/2005 4:55:07 AM >>>
We have started to see a hazy/cloudiness to our alcian blue PAS slides.
 We
don't see this on any other slide/stain.  We use the same slides,
reagents,
and procedure that we have always used.  The stain works fine and the
haze
is on the entire slide, not just the tissue.  We routinely use the
Fisherbrand red control slides and have tried other slides and still
have
the same problem.  I use the Poly Scientific reagents (alcian blue ph
2.5)
and have switched them all out for new  reagents but still have the
haze.
We have switched out, altered, and tried everything I can think of.  I
am
totally baffled.  Hope you folks have some ideas.

Tom Mc Nemar HT(ASCP)
Histology Supervisor
Licking Memorial Hospital
Newark, Ohio 43055


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