Reference to Gayle on RE: [Histonet] another background / non-specific staining problem

Wed Feb 2 09:28:28 CST 2005

Rebekah,

Sorry folks but this is not a recommendation from me.

I have never done this!

At 08:42 PM 2/1/2005, you wrote:
>I had a similar problem when staining non-perfused sheep placenta with 
>eNOS and someone (I think it was Gayle) on Histonet suggest putting my 
>slides in 1% NaOH (the other 99 being ethanol) solution. I've been putting 
>my slides in 1% NaOH for 15 min after the peroxide and it has worked well.

Gayle Callis
MT,HT,HTL(ASCP)
Research Histopathology Supervisor
Veterinary Molecular Biology
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610
406 994-6367 (lab with voice mail)
406 994-4303 (FAX)