[Histonet] CD-20 nuclear staining....

[Richard Cartun]

I have never seen nuclear staining with CD20 (clone L26).  Which
pretreatment and detection system are you using?

Richard

Richard W. Cartun, Ph.D.
Director, Immunopathology & Histology
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 545-1596
(860) 545-0174 Fax

>>> Brandon Stokes <cuttingedgehistology <@t> yahoo.com> 02/01/05 09:26PM
>>>
Hello fellow histo-netters,
 
I was wondering if anyone else has ever experienced nuclear staining
occuring when using the CD-20 antibody.  The tonsil control will look
great....then you look at the patient tissue(almost always skin for us)
and some of the nuclei of the keratinocytes in the epidermis and even
some nuclei in the dermis might light up bright red(we use AEC).  You
can actually see the nuclear detail very well.  This problem doesn't
happen all the time so I am not sure what it could be.  
 
Any advice would be greatly appreciated.
 
 
Brandon Stokes, HT(ASCP)
Lab Manager
Cutting Edge Histology Services, LLC
 

		
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