[Histonet] Problem with mucicarmine stain

Bonner, Janet Janet.Bonner <@t> FLHOSP.ORG
Tue Feb 1 16:22:19 CST 2005

Your control may be old - sometimes, depending on where you are and the
conditions it's stored at, the reactivity diminishes.   Janet

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
To: Histonet (E-mail); Chung, Luong
Sent: 2/1/2005 4:11 PM
Subject: Re: [Histonet] Problem with mucicarmine stain

        A few more questions before we can help.....
  a.. Are you using tap water or distilled to make the working
  b.. How long are you leaving it in the mucicarmine? 
  c.. Is this done at room temp? 
  d.. Do you use hematoxylin?
  ----- Original Message ----- 
  From: Chung, Luong<mailto:lchung <@t> ppmh.org> 
  To: Histonet (E-mail)<mailto:histonet <@t> lists.utsouthwestern.edu> 
  Sent: Tuesday, February 01, 2005 2:04 PM
  Subject: [Histonet] Problem with mucicarmine stain


  When we do mucicarmine (Richar/Allen pre-made) according to our
protocol with dilution of one to four.  We counterstain with tretrazine.
  We use appendix as a control and the stain turn out to be very pale.
Is there any remedy for this?
  It used to work very well, but not lately.


  Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu<mailto:Histonet <@t> lists.utsouthwestern.e
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu

The information contained in this message may be privileged and/or
confidential and protected from disclosure.  If the reader of this message
is not the intended recipient or agent responsible for delivering this
message to the intended recipient, you are hereby notified that any
dissemination, distribution or copying of this communication is strictly
prohibited.  If you have received this communication in error, please notify
the sender immediately by replying to this message and deleting the material
from any computer. 

More information about the Histonet mailing list