[Histonet] de Olmos staining

[John Kiernan]

The reference is:

De Olmos JS, Beltramino CA, De Lorenzo SDO (1994) 
Use of an amino-cupric-silver technique for the detection 
of early and semiacute neuronal degeneration caused by 
neurotoxicants, hypoxia, and physical trauma. 
Neurotoxicology and Teratology 16: 545-561.

For a complicated silver method like this one, it's 
important to read the original, not just a list of
instructions. You can get the Abstract of the De Olmos
paper on line at

http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?holding=npg&cmd=Retrieve&db=PubMed&list_uids=7532272&dopt=Abstract

but this doesn't say how to do the method. For that
there's no alternative to a trip to the library.
-- 
-------------------------------
John A. Kiernan
Department of Anatomy and Cell Biology
The University of Western Ontario
London,   Canada   N6A 5C1
   kiernan[AT]uwo.ca
   http://publish.uwo.ca/~jkiernan/
   http://instruct.uwo.ca/anatomy/530/index.htm
_______________________________
Martina Urbanek wrote:
> 
> Hello, Everyone,
> 
> Does anyone have a detailed protocol for de Olmos amino-cupric-silver stain
> which works on paraffin sections (does it even work on paraffin?)? We work with
> mice brains which are fixed in 10% formalin, but not perfused and we are
> looking for a technique to stain degenerated neuronal cells.
> Your help is greatly appreciated.
> 
> Martina Urbanek
> 
> Ms. Martina Urbanek
> Forschungslabor der
> Klin.Abt. für Neonatologie
> neonatal neuroscience research laboratory
> Med. University Innsbruck
> Innrain 66, 4th floor
> A-6020 Innsbruck
> Tel. +43 (0)512 504 27755/27765
> Fax: +43 (0)512 504 27766
> Email: Martina.Urbanek <@t> uibk.ac.at
> 
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet