[Histonet] de Olmos staining

John Kiernan jkiernan <@t> uwo.ca
Tue Feb 1 16:13:20 CST 2005

The reference is:

De Olmos JS, Beltramino CA, De Lorenzo SDO (1994) 
Use of an amino-cupric-silver technique for the detection 
of early and semiacute neuronal degeneration caused by 
neurotoxicants, hypoxia, and physical trauma. 
Neurotoxicology and Teratology 16: 545-561.

For a complicated silver method like this one, it's 
important to read the original, not just a list of
instructions. You can get the Abstract of the De Olmos
paper on line at


but this doesn't say how to do the method. For that
there's no alternative to a trip to the library.
John A. Kiernan
Department of Anatomy and Cell Biology
The University of Western Ontario
London,   Canada   N6A 5C1
Martina Urbanek wrote:
> Hello, Everyone,
> Does anyone have a detailed protocol for de Olmos amino-cupric-silver stain
> which works on paraffin sections (does it even work on paraffin?)? We work with
> mice brains which are fixed in 10% formalin, but not perfused and we are
> looking for a technique to stain degenerated neuronal cells.
> Your help is greatly appreciated.
> Martina Urbanek
> Ms. Martina Urbanek
> Forschungslabor der
> Klin.Abt. für Neonatologie
> neonatal neuroscience research laboratory
> Med. University Innsbruck
> Innrain 66, 4th floor
> A-6020 Innsbruck
> Tel. +43 (0)512 504 27755/27765
> Fax: +43 (0)512 504 27766
> Email: Martina.Urbanek <@t> uibk.ac.at
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