[Histonet] intranasal delivery dye tracer

[Geoff McAuliffe]

    Hmmm. If the "practice" solutions work, does that mean that the 
fluor-tagged marker will work? I think not. I would try something of the 
same size, molecule wise, otherwise the practice may be for naught. If 
you want to get substance X into the CNS, I don't understand practicing 
with substance Y. Also, it sounds like your boss is assuming that the 
tracer will be picked up by olfactory neurons and transported to a 
particular target in the CNS? If the marker gets into the circulatory 
system (via the olfactory mucosa), the blood brain barrier will still be 
in the way.

Geoff

Surita Banwait wrote:

>Hello
>
>We are interested in trying a new method of delivery in mice (intranasal
>delivery) to avoid dealing with the blood brain barrier.  Eventually a
>fluorescence-tagged marker will be delivered and analyzed.  Before we do
>this however, my PI is interested in delivering a few different
>solutions (preferably a colored dye) that we can track through the mouse
>brain.  Are there any suggestions as to some solutions we might try?  
>
> 
>
> 
>
>Thanks for your consideration,
>
>Surita   
>
> 
>
>Surita Banwait
>
>Morphology Core
>
>Research Associate II
>
>Buck Institute for Age Research
>
>8001 Redwood Blvd.
>
>Novato, CA 94945
>
>415-209-2221
>
>sbanwait <@t> buckinstitute.org
>
> 
>
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Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
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mcauliff <@t> umdnj.edu
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