[Histonet] Re: Mouse liver fixation
gcallis <@t> montana.edu
Tue Feb 1 09:32:38 CST 2005
We had terrible problems with mouse liver when whole livers were immersed
in only 50 ml neutral buffered formalin and left to sit around. The tissue
brought in a week later was NOT fixed internally, it was still red. If
you can perfuse the animal, dissect out liver and immerse into formalin
1:20 volume. Or fix for a day, take liver out, slice it and return to
Liver is very homogenous, dense and surprisingly takes a longer time to fix
than mouse lung. If liver is dissected out, bread slicing it for better
fixation is advisable. Slices are embedded cut sides down unless you want
to embed a whole liver - a rather large chunk of tissue for such a little
critter. If you plan on a whole liver, be sure to change the fixative
after a day or so to replenish the formalin and this is advisable with
sliced liver too.
Whatever you do, make sure fixative can reach internal parts of liver or
you get some autolyzed tissue.
At 05:12 AM 2/1/2005, you wrote:
>Thanks for your great advice last time on fixing mouse lungs. You'll be
>glad to know we've got it working and have some nice H+E slides and the
>immunos will be staring soon.
>Can you help again? My boss has decided to look at the mouse's liver as
>well (poor thing!) and I'd be grateful if you have any tips on fixing
>liver. Can you just remove the liver and place it in a vial of formalin or
>does it need perfused etc?
>Looking forward to hearing from you all again.
>Clinical Research Fellow
>andrew.macduff <@t> ed.ac.uk
>Histonet mailing list
>Histonet <@t> lists.utsouthwestern.edu
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