[Histonet] RE: aqueous mounting medium

[Amanda MacFarlane]

This is good to know. I was under the impression from what I've read that
the chromogenic product of beta-gal was soluble in xylene, thus the need
for aqueous mounting medium.
Thanks,
Amanda
>
> Hi Amanda,
> From my experiments with X-gal as chromogen for IHC techniques it appeared
> that the blue-greenish reaction product (with X-gal + ferro/ferricyanide)
> is very stable and survives any mounting medium. You may dehydrate and
> mount organically.
> Chris van der Loos, PhD
> Dept. of Pathology
> Academical Medical Center M2-230
> Meibergdreef 9
> NL-1105 AZ Amsterdam
> The Netherlands
> phone:  +31 20 5665631
> fax:    +31 20 6960389
> e-mail: c.m.vanderloos <@t> amc.uva.nl
>
> ----- Original Message -----  From  "Amanda MacFarlane"   Date  Mon, 31
> Jan 2005 11:38:24 -0500 (EST)  To  Histonet <@t> lists.utsouthwestern.edu
> Subject  [Histonet] aqueous mounting medium
> I have stained whole tissues for beta-galactosidase (X-gal) and am now
> cryosectioning the tissues. I have been fixing the sections in 4% PFA
> overnight at 4 degrees Celsius and cover slipping with 70% glycerol.
> Coverslipping with glycerol is a time-consuming pain in the butt so I've
> checked out glycerol vinyl alcohol aqueous (GVA) mounting solution (Zymed)
> and Supermount aqueous mounting medium (Biogenex) as alternatives. It
> appears that these products have not been tested with beta-gal staining,
> so I'm wondering if people use these products and/or  what people
> recommend. Thanks.
>
>
> Amanda MacFarlane, PhD
> Division of Nutritional Sciences
> Cornell University
>


Amanda MacFarlane
Division of Nutritional Sciences
Cornell University