[Histonet] RE: aqueous mounting medium

[C.M. van der Loos]

   Hi Amanda,

   From my experiments with X-gal as chromogen for IHC techniques it
   appeared that the blue-greenish reaction product (with X-gal +
   ferro/ferricyanide) is very stable and survives any mounting medium.
   You may dehydrate and mount organically.

   Chris van der Loos, PhD
   Dept. of Pathology
   Academical Medical Center M2-230
   Meibergdreef 9
   NL-1105 AZ Amsterdam
   The Netherlands

   phone:  +31 20 5665631
   fax:    +31 20 6960389
   e-mail: [1]c.m.vanderloos <@t> amc.uva.nl


   ----- Original Message -----
      From  "Amanda MacFarlane" <am458 <@t> cornell.edu>
      Date  Mon, 31 Jan 2005 11:38:24 -0500 (EST)
        To  Histonet <@t> lists.utsouthwestern.edu
   Subject  [Histonet] aqueous mounting medium

   I have stained whole tissues for beta-galactosidase (X-gal) and am now
   cryosectioning the tissues. I have been fixing the sections in 4% PFA
   overnight at 4 degrees Celsius and cover slipping with 70% glycerol.
   Coverslipping  with  glycerol  is a time-consuming pain in the butt so
   I've
   checked  out  glycerol  vinyl  alcohol aqueous (GVA) mounting solution
   (Zymed)
   and Supermount aqueous mounting medium (Biogenex) as alternatives. It
   appears  that  these  products  have  not  been  tested  with beta-gal
   staining,
   so I'm wondering if people use these products and/or  what people
   recommend. Thanks.
   Amanda MacFarlane, PhD
   Division of Nutritional Sciences
   Cornell University

References

   1. mailto:c.m.vanderloos <@t> amc.uva.nl