[Histonet] Millers Elastin Stain HELP

S Ladd sladd <@t> hsc.usf.edu
Tue Feb 1 07:39:00 CST 2005

There's a great article on elastic staining in Sakura's Histologic Vol.
XXXII No. 1, May 2000, page 12 entitled "Elastic Tissue Staining in Human
Skin." That's where I found out that you can buy Miller's from Rowley
Biochemical, 10 Electronics Ave. Danvers, MA 01923 978-739-4883 or
www.rowleybio.com. A couple years ago it cost $55 for a pint. As for your
questions 2 and 3, I have no idea.

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu]On Behalf Of Celebre
Sent: Tuesday, February 01, 2005 8:14 AM
To: 'histonet <@t> lists.utsouthwestern.edu'
Subject: [Histonet] Millers Elastin Stain HELP

Good morning all....

Our lab makes our own Millers solution, and I think it's just for plain old
punishment because if anyone has had to make it they understand what I
mean... Normally the stain works beautifully, but since we've run out of
'Victoria Blue 4R' and have used 'Victoria Blue B' as a substitute the stain
doesn't seem to be working too well. The reason why we substituted because
Victoria Blue 4R is no longer availalbe..

What I'm asking all you histology guru's out there...
1. Does anybody know of a company that sells pre-made Millers?
2. Does anybody know where we can purchase Victoria Blue 4R?
3. Does anybody know if/why Victoria Blue B can be used as a substitute?


Julia Celebre MLT
Anatomic Pathology
Hamilton General Hospital
905-527-0271  ext46145
email: celebrej <@t> hhsc.ca

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