[Histonet] Responses to mouse liver tissue processing

[Luis Chiriboga]

everyone suggested changing the processing schedule (I would if I had could)
+  permutations on cold water soaking to get good sections.
Thanks to everyone who responded.
Peace & Happy Holidays to all
Luis

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu]On Behalf Of Luis
Chiriboga
Sent: Wednesday, December 21, 2005 9:54 AM
To: Histonet
Subject: [Histonet] Mouse liver tissue processing


Hi everyone
We are having a real serious problem getting good sections from mouse
livers.  The tissue curls and shreds out of the block as soon as it comes in
contact with the knife, end up with a section that has the exact outline of
the tissue but no tissue. The tissue consistency could best be described as
"dry?" Interestingly, it is only the normal livers that have this problem.
We have tried sectioning thick, thin, no soak, cold soak......but out of
ideas. Anyone have any suggestions? Unfortunately we have no control over
the tissue processing, but perhaps can convince to change.
Thanks Luis

Happy Holidays to all!!
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