[Histonet] Rat eyes in paraffin

A. Erickson andrae <@t> u.washington.edu
Mon Dec 19 13:29:36 CST 2005

Hi all,
I have a problem when blocking the rat eyes in paraffin. The eye tend to 
colapse and what I need is the round eye.

My paraffin procedure is fix the eyes for 15 minute in 70%, 80%, 95%, 
100%, mix 100%+Toluene, 2X Toluene. Then 30 minutein mix 
Toluene+Paraffin, Paraffin, Paraffin+vaccum.

Most of the eyes were colaps. Is anyone has another protocol to prevent 
the colapsing of the eyes?

Thank you so much.

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