[Histonet] Rat eyes in paraffin
andrae <@t> u.washington.edu
Mon Dec 19 13:29:36 CST 2005
I have a problem when blocking the rat eyes in paraffin. The eye tend to
colapse and what I need is the round eye.
My paraffin procedure is fix the eyes for 15 minute in 70%, 80%, 95%,
100%, mix 100%+Toluene, 2X Toluene. Then 30 minutein mix
Toluene+Paraffin, Paraffin, Paraffin+vaccum.
Most of the eyes were colaps. Is anyone has another protocol to prevent
the colapsing of the eyes?
Thank you so much.
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