[Histonet] sealing slides with diluted permount

[Malcolm McCallum]

I found this.
http://www.clontech.com/clontech/techinfo/faqs/LivingColors/fixation.shtml

Q What is the best method of sealing coverslips? 

A We recommend sealing coverslips with molten agarose or rubber cement. We do not recommend using nail polish, because GFP is very sensitive to some nail polishes. However, when using ProLong Antifade from Molecular Probes (www.probes.com <http://www.probes.com/media/pis/mp07481.pdf> ) no further sealing is necessary.

 
Malcolm L. McCallum
Assistant Professor
Department of Biological Sciences
Texas A&M University Texarkana
2600 Robison Rd.
Texarkana, TX 75501
O: 1-903-233-3134
H: 1-903-791-3843
Homepage: https://www.eagle.tamut.edu/faculty/mmccallum/index.html
 

________________________________

From: histonet-bounces <@t> lists.utsouthwestern.edu on behalf of Caroline Bass
Sent: Mon 12/19/2005 12:51 PM
To: Histonet (E-mail)
Subject: [Histonet] sealing slides with diluted permount



Hello,

I have some slides of GFP expressing tissue.  I am using the prolong 
gold mounting media from Molecular Probes to mount the coverslips but 
now I have to seal them.  I have read that the best way to do this is 
with diluted permount.  Unfortunately I have never sealed slides 
before and am having a hard time visualizing the process.

What is a good starting dilution for the permount?  I was thinking 
1:10 with xylenes to start, but this is just a guess.  How exactly do 
you apply the permount?  Can you just pipet it around the edges or do 
you need to brush it on in some manner?  Does it have to be flush 
with the coverslip or can you have some buildup?

Any advice would be appreciated.

Thanks,

Caroline Bass

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