[Histonet] sealing slides with diluted permount
Malcolm.McCallum <@t> tamut.edu
Mon Dec 19 13:04:57 CST 2005
I found this.
Q What is the best method of sealing coverslips?
A We recommend sealing coverslips with molten agarose or rubber cement. We do not recommend using nail polish, because GFP is very sensitive to some nail polishes. However, when using ProLong Antifade from Molecular Probes (www.probes.com <http://www.probes.com/media/pis/mp07481.pdf> ) no further sealing is necessary.
Malcolm L. McCallum
Department of Biological Sciences
Texas A&M University Texarkana
2600 Robison Rd.
Texarkana, TX 75501
From: histonet-bounces <@t> lists.utsouthwestern.edu on behalf of Caroline Bass
Sent: Mon 12/19/2005 12:51 PM
To: Histonet (E-mail)
Subject: [Histonet] sealing slides with diluted permount
I have some slides of GFP expressing tissue. I am using the prolong
gold mounting media from Molecular Probes to mount the coverslips but
now I have to seal them. I have read that the best way to do this is
with diluted permount. Unfortunately I have never sealed slides
before and am having a hard time visualizing the process.
What is a good starting dilution for the permount? I was thinking
1:10 with xylenes to start, but this is just a guess. How exactly do
you apply the permount? Can you just pipet it around the edges or do
you need to brush it on in some manner? Does it have to be flush
with the coverslip or can you have some buildup?
Any advice would be appreciated.
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