[Histonet] Re: CD4 and CD8 mouse FFPE tissues

[patsy ruegg]

Jamie,
I agree with you on this.  I gave up trying to do cd4 and cd8 on ffpe mouse
tissue and do it only as frozens now.  I am one that can't even get them to
work on human ffpe tissues, people have told me their methods but when I try
I fail even with human tissues with these t cell subsets.
Patsy 

Patsy Ruegg, HT(ASCP)QIHC
IHCtech
12635 Montview Blvd. #216
Aurora, CO 80010
720-859-4060
fax 720-859-4110
pruegg <@t> ihctech.net
www.ihctech.net 

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Jamie E
Erickson
Sent: Thursday, December 15, 2005 2:13 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Re: CD4 and CD8 mouse FFPE tissues

HI Reinhard,
              I have tried many and I repeat many times to get CD4 and CD8 
to work in FFPE tissues mostly with the purified monoclonals antibody from 
BD biosciences and have failed. I have been doing mouse IHC for 12 years 
and have come to the conclusion after many try's and conversations with 
others in the field (Gayle Callis and others) that for CD4 and CD8 just 
doesn't work for mouse FFPE IHC. We do research and I'm going to start 
doing more frozen mouse work. Frozens are faster and you don't have to 
beat your head against the wall to think of more antigen retrieval to try. 
This may not be everyone's experience but for me I've had enough FFPE 
CD4/CD8 and others..

 That's my opinion for what it worth.

_______________________________
Jamie Erickson
Sr. Research Associate 
Department: DSMP
Abbott Bioresearch Center
100 Research Drive
Worcester, MA 01605-4341
508-688-3134
FAX: 508-793-4895
e-mail: jamie.erickson <@t> abbott.com
 
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