[Histonet] Re: CD4 and CD8 mouse FFPE tissues
Jamie E Erickson
jamie.erickson <@t> abbott.com
Thu Dec 15 15:13:29 CST 2005
I have tried many and I repeat many times to get CD4 and CD8
to work in FFPE tissues mostly with the purified monoclonals antibody from
BD biosciences and have failed. I have been doing mouse IHC for 12 years
and have come to the conclusion after many try's and conversations with
others in the field (Gayle Callis and others) that for CD4 and CD8 just
doesn't work for mouse FFPE IHC. We do research and I'm going to start
doing more frozen mouse work. Frozens are faster and you don't have to
beat your head against the wall to think of more antigen retrieval to try.
This may not be everyone's experience but for me I've had enough FFPE
CD4/CD8 and others..
That's my opinion for what it worth.
Sr. Research Associate
Abbott Bioresearch Center
100 Research Drive
Worcester, MA 01605-4341
e-mail: jamie.erickson <@t> abbott.com
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