[Histonet] Re: CD4 and CD8 mouse FFPE tissues

[Jamie E Erickson]

HI Reinhard,
              I have tried many and I repeat many times to get CD4 and CD8 
to work in FFPE tissues mostly with the purified monoclonals antibody from 
BD biosciences and have failed. I have been doing mouse IHC for 12 years 
and have come to the conclusion after many try's and conversations with 
others in the field (Gayle Callis and others) that for CD4 and CD8 just 
doesn't work for mouse FFPE IHC. We do research and I'm going to start 
doing more frozen mouse work. Frozens are faster and you don't have to 
beat your head against the wall to think of more antigen retrieval to try. 
This may not be everyone's experience but for me I've had enough FFPE 
CD4/CD8 and others..

 That's my opinion for what it worth.

_______________________________
Jamie Erickson
Sr. Research Associate 
Department: DSMP
Abbott Bioresearch Center
100 Research Drive
Worcester, MA 01605-4341
508-688-3134
FAX: 508-793-4895
e-mail: jamie.erickson <@t> abbott.com