[Histonet] Mouse Autoradiography of fixed frozen tissue

Sebree Linda A. la.sebree <@t> hosp.wisc.edu
Fri Dec 16 13:53:15 CST 2005

Its been a long time Jamie, but when I did autoradiography on frozen
rabbit heart, I simply cut the frozen sections in a dark room, air-dried
and dipped in emulsion.  Then I sealed them in a light-tight box to

Linda A. Sebree
University of Wisconsin Hospital & Clinics
IHC/ISH Clinical & Research Laboratory
600 Highland Ave.
Madison, WI 53792
FAX:  (608)262-7174

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Jamie E
Sent: Friday, December 16, 2005 1:20 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Mouse Autoradiography of fixed frozen tissue

Hello everyone,
                          I was hoping that I can get some assistance on
project I'm going to do. An Investigator here would like to look at 
radiolabeled mouse Sm. intestines for there specific radiolabeled
My problem is that I don't want to use our only processor to process 
radioactive material ( I 125 or H3, not sure yet) My other histology 
colleges may not like me so much if I did that.
       So I plan on fixing the material in 10% NBF (24-48 hours),
in water (Blot)  then cryopreserving them (LN2 Isopentane). This way I
Cryosection and decontaminate the less used cryostat.  Next I  wash in 
water and lastly dipping slides in Photographic Emulsion and wait, and 
wait....My question is, has anyone done this that could help me out with

the finer details and will this be OK?  I've done this with paraffin 
samples dip wait 2,4,7,14, 21, etc days for a signal, but not with 

If anyone has done this and could help me out I'd really appreciate it..



Jamie Erickson
Sr. Research Associate 
Department: DSMP
Abbott Bioresearch Center
100 Research Drive
Worcester, MA 01605-4341
FAX: 508-793-4895
e-mail: jamie.erickson <@t> abbott.com
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