[Histonet] 10% formalin Fixation time for Non-Human Primate

Roger Moretz stamptrain <@t> yahoo.com
Fri Dec 16 09:53:18 CST 2005


While penetration is usually complete by 24 hours,
fixation in formalin (formaldehyde) is not considered
to be complete until about 48 to 72 hours.  We have
found that 48hours is the minimum time we can use to
get optimum preservation.  

Roger Moretz, Ph.D.
Dept of Toxicology
Boehringer Ingelheim Pharmaceuticals, Inc.
Rigdefield, CT

--- Sharon E Willman <sharon.willman <@t> bms.com> wrote:

> Hi,
> I am trying to find the fixation time in 10% NBF
> before the decal 
> process (13% formic acid)
> for non-human primate, canine, and rodent nasal
> cavities.  I would 
> appreciate any help someone could offer.
> Thanks,
> Sharon Willman
> 
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