[Histonet] SDH protocol

[Lewis, Sarah]

Fellow Neuromuscular Labs!!
I am in need of a Great SDH protocol.  I have been struggling with this stain for a long time.  I purchase new NBT and it works pretty well for a few weeks.  Then is starts fading into nothing within 1month.   I use a protocol of on the WashU website (St. Louis MO). It works better than any other I have tried... but still very pale, However my pathologist said that the SDH's in the past have always been more blue.  Mine come out purple/ pink.  I have also used the protocol from past histologist in this lab with no luck.  I must be missing some small technique that isn't written in the protocol.  Please help!!!       

Sarah E. Lewis 
Histotechnician 
Childrens Research 
Center for Gene Therapy 
700 Childrens Dr Rm WA3112 
Columbus OH 43205 
(614)-722-2204 
LewisS <@t> ccri.net