[Histonet] IHC controls for Cytology
Rogerson Kemlo (ELHT) Pathology
Kemlo.Rogerson <@t> elht.nhs.uk
Wed Dec 14 09:14:07 CST 2005
Aren't controls supposed to be treated exactly the same as the test? So
unless you treat them exactly the same you will never know if you have a
false negative and I'm not bright enough to know if you could have false
We have very recently begun to destain pap stained smears as well as
unstained cytology smears, and run IHC.
Because my Docs are happy with the results, I anticipate receiving more
of these types of smears to run.
I only have FFPE tissue in stock to use as control, so I give them the
slides with the disclaimer that the control has been treated differently
than the smear.
This seems to be acceptable as long as they can detect some positive
stained cells (TTF, PanCk, WT-1 Mart-1 for example). But if the smear
is negative, how can I be sure it's a true negative? My antibodies
aren't really titered for smears....
I would appreciate some comments from anyone who is running a
standardized protocol for smears. Do you have a set of smears you can
use as controls? I have access to our molecular lab and there is
potential to make my own cell cultured controls, what do you think?
Becky Orr CLA,HT(ASCP)
Evanston Northwestern Healthcare
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
More information about the Histonet