[Histonet] IHC controls for Cytology
Sebree Linda A.
la.sebree <@t> hosp.wisc.edu
Wed Dec 14 09:08:23 CST 2005
When we were doing IHC on cytospins and ThinPreps, we used frozen tissue
sections that had been acetone fixed the same way as the cytology preps.
We were able to optimize our antibodies using the frozen sections and
the protocols developed always worked for the cytology preps as well.
We figured frozens were a much closer preparation methodology than
paraffin sections and our pathologists were happy with the compromise we
came up with.
Linda A. Sebree
University of Wisconsin Hospital & Clinics
IHC/ISH Clinical & Research Laboratory
600 Highland Ave.
Madison, WI 53792
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Orr,
Sent: Wednesday, December 14, 2005 8:44 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] IHC controls for Cytology
We have very recently begun to destain pap stained smears as well as
unstained cytology smears, and run IHC.
Because my Docs are happy with the results, I anticipate receiving more
of these types of smears to run.
I only have FFPE tissue in stock to use as control, so I give them the
slides with the disclaimer that the control has been treated differently
than the smear.
This seems to be acceptable as long as they can detect some positive
stained cells (TTF, PanCk, WT-1 Mart-1 for example). But if the smear
is negative, how can I be sure it's a true negative? My antibodies
aren't really titered for smears....
I would appreciate some comments from anyone who is running a
standardized protocol for smears. Do you have a set of smears you can
use as controls? I have access to our molecular lab and there is
potential to make my own cell cultured controls, what do you think?
Becky Orr CLA,HT(ASCP)
Evanston Northwestern Healthcare
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