[Histonet] combined FISH/IHC protocol

[Kim Lopez]

Hi all,

I wanna do combined FISH and IHC on frozen rat brain using a rat
y-chromosome probe and Ki67.  I've already found one paper that has
done something similar on mouse brains using a mouse y-chromosome
probe and anti-GFP.  It seemed to work well for them and I'm going
to try and replicate using their protocol (Donadoni C, J Histochem
& Cytochem, 2004).  I was just wondering if anybody else has any
experience with this.  I will be very grateful for any tips or
advice.

Kim