[Histonet] temperature for sectioning fixed liver

Caroline Bass cbass <@t> bidmc.harvard.edu
Tue Dec 13 14:06:32 CST 2005


I am going to section 4% formaldehyde fixed liver blocks on a  
cryostat this afternoon.  It is the first time that I have sectioned  
this particular tissue in a cryostat.  Could someone recommend a good  
starting temperature for specimen and chamber?  I have a  
recommendation of -17 for frozen tissue, but is this good for fixed  
as well?

I am going to try 10 um sections initially.


Caroline Bass

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