[Histonet] RE: Histonet Digest, Vol 25, Issue 16
Featherstone, Annette
AFeatherstone <@t> KaleidaHealth.Org
Tue Dec 13 12:39:38 CST 2005
We need a different protocol for Glucose Tolerance Test. Does anyone out
there have one they would be willing to email me or fax? My fax # is 716
8591853. thanks in advance
Annette Featherstone HT?MLT
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
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Sent: Tuesday, December 13, 2005 13:05
To: histonet <@t> lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 25, Issue 16
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Today's Topics:
1. RE: Emergency showers in Lab (Patsy Ruegg)
2. RE: bone protocal (Patsy Ruegg)
3. Re: Emergency showers in Lab (Rene J Buesa)
4. Re: Emergency showers in Lab (Judith LaDuc)
5. RE: Emergency showers in Lab (Poteete, Jacquie A.)
6. Re: Emergency showers in Lab (Ada Feldman)
7. IHC Contractor (Jackie M O'Connor)
8. IHC on coverglass (Steven Coakley)
9. Re: IHC on coverglass (Loralei Dewe)
10. RE: IHC on coverglass (Sebree Linda A.)
11. rat spinal cord longitudinal sections (Caroline Bass)
12. RE: rat spinal cord longitudinal sections (Monfils, Paul)
13. DAB info disposabel/neutralization/tratment (crs grupo)
14. Re: DAB info disposabel/neutralization/tratment (Rene J Buesa)
15. anti-mouse PR primary Ab (Vernon Dailey)
16. RE: Emergency showers in Lab (Smith, Allen)
17. FFPE CD31 on mouse (Jackie M O'Connor)
18. RE: FFPE CD31 on mouse (Koelling, Ray)
19. Protocols that have worked using BD PharminGen monoclonal Re:
[Histonet] FFPE CD31 on mouse (Gayle Callis)
20. Florida License (DDDeltour <@t> mar.med.navy.mil)
21. Another Histonet Archive CD31 for murine FFPE tissue
(Gayle Callis)
----------------------------------------------------------------------
Message: 1
Date: Mon, 12 Dec 2005 11:13:18 -0700
From: "Patsy Ruegg" <pruegg <@t> ihctech.net>
Subject: RE: [Histonet] Emergency showers in Lab
To: "'Weems, Joyce'" <JWEEMS <@t> sjha.org>, "'Jill Cox'"
<jcox90 <@t> yahoo.com>, "'Histonet <@t> Lists. Utsouthwestern. Edu'"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <200512121813.jBCID0N1019734 <@t> chip.viawest.net>
Content-Type: text/plain; charset="us-ascii"
We do.
Patsy Ruegg, HT(ASCP)QIHC
IHCtech, LLC
Fitzsimmons BioScience Park
12635 Montview Blvd. Suite 216
Aurora, CO 80010
P-720-859-4060
F-720-859-4110
wk email pruegg <@t> ihctech.net
web site www.ihctech.net
This email is confidential and intended solely for the use of the Person(s)
('the intended recipient') to whom it was addressed. Any views or opinions
presented are solely those of the author. It may contain information that is
privileged & confidential within the meaning of applicable law. Accordingly
any dissemination, distribution, copying, or other use of this message, or
any of its contents, by any person other than the intended recipient may
constitute a breach of civil or criminal law and is strictly prohibited. If
you are NOT the intended recipient please contact the sender and dispose of
this e-mail as soon as possible.
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Weems, Joyce
Sent: Monday, December 12, 2005 9:07 AM
To: Jill Cox; Histonet <@t> Lists. Utsouthwestern. Edu
Subject: RE: [Histonet] Emergency showers in Lab
We do.
Joyce Weems
Pathology Manager
Saint Joseph's Hospital of Atlanta
404-851-7376
404-851-7831 - fax
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu]On Behalf Of Jill Cox
Sent: Monday, December 12, 2005 10:49 AM
To: Histonet <@t> Lists. Utsouthwestern. Edu
Subject: [Histonet] Emergency showers in Lab
Hello Histonetters!!
Just wondering if anyone knows about regs for an emergency shower in a
Pathology lab? I can't find anything in CAP checklist or OSHA on this. Is
there somewhere else I can look? Who all has showers? Thanks in advance,
Jill
Jill Cox HT (ASCP)
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information is strictly prohibited. If you have received this communication
in error, please notify us immediately by replying to the message and
deleting it from your computer. Thank you. Saint Joseph's Health System,
Inc.
------------------------------
Message: 2
Date: Mon, 12 Dec 2005 11:14:27 -0700
From: "Patsy Ruegg" <pruegg <@t> ihctech.net>
Subject: RE: [Histonet] bone protocal
To: "'caron fournier'" <caron_fournier <@t> yahoo.ca>,
<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <200512121814.jBCIE8N1020089 <@t> chip.viawest.net>
Content-Type: text/plain; charset="us-ascii"
Why don't you embed in PMMA?
Patsy
Patsy Ruegg, HT(ASCP)QIHC
IHCtech, LLC
Fitzsimmons BioScience Park
12635 Montview Blvd. Suite 216
Aurora, CO 80010
P-720-859-4060
F-720-859-4110
wk email pruegg <@t> ihctech.net
web site www.ihctech.net
This email is confidential and intended solely for the use of the Person(s)
('the intended recipient') to whom it was addressed. Any views or opinions
presented are solely those of the author. It may contain information that is
privileged & confidential within the meaning of applicable law. Accordingly
any dissemination, distribution, copying, or other use of this message, or
any of its contents, by any person other than the intended recipient may
constitute a breach of civil or criminal law and is strictly prohibited. If
you are NOT the intended recipient please contact the sender and dispose of
this e-mail as soon as possible.
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of caron
fournier
Sent: Monday, December 12, 2005 9:02 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] bone protocal
Hi All:
I am new to a position where I am doing undecalcified bone sectioning and
grinding. We have a project coming up that I need some imput on....we will
be fixing and embedding sheep spine with implants but the implants are PMMA
and I need to know if there is a protocal that I can follow that will embedd
these in a resin hard enough to grind but that will not damage the existing
PMMA of the implant. Can anyone help?
Caron
Caron Fournier, BSc, R.T.
Department of Orthopaedics,
Division of Orthopaedic Engineering Research, U.B.C.
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------------------------------
Message: 3
Date: Mon, 12 Dec 2005 10:27:03 -0800 (PST)
From: Rene J Buesa <rjbuesa <@t> yahoo.com>
Subject: Re: [Histonet] Emergency showers in Lab
To: Jill Cox <jcox90 <@t> yahoo.com>, "Histonet <@t> Lists. Utsouthwestern. Edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <20051212182703.93891.qmail <@t> web61215.mail.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1
Jill:
I had always have a shower in the lab, and that is what you want to have
if handling chemicals (of any type) that could spill over you. This is why
there are also showers in the Chemistry labs. It is a safety requirement in
Florida.
Rene J.
Jill Cox <jcox90 <@t> yahoo.com> wrote:
Hello Histonetters!!
Just wondering if anyone knows about regs for an emergency shower in a
Pathology lab? I can't find anything in CAP checklist or OSHA on this. Is
there somewhere else I can look? Who all has showers? Thanks in advance,
Jill
Jill Cox HT (ASCP)
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http://lists.utsouthwestern.edu/mailman/listinfo/histonet
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Message: 4
Date: Mon, 12 Dec 2005 13:51:26 -0500
From: "Judith LaDuc" <jladuc <@t> trudeauinstitute.org>
Subject: Re: [Histonet] Emergency showers in Lab
To: <histonet <@t> lists.utsouthwestern.edu>, <jcox90 <@t> yahoo.com>
Message-ID: <s39d807f.017 <@t> trudeauinstitute.org>
Content-Type: text/plain; charset=US-ASCII
Hi Jill,
You can find the info at www.osha.gov if you look through the formaldehyde
standard. I can't remember it off the top of my head... 1410 - ?
There are many other safety organizations out there. I have a link below
that is using some ASNI information that may be helpful to you. A Google or
other internet search will bring up tons of safety shower hits for you as
well. I do remember that they should be able to be operated with one hand
and stay on after activation. It is also important to flush them (eye
washes) weekly if they are attached to plumbing to avoid stagnant solutions.
I heard at a recent seminar that you may need to arrange for tepid (warm)
water as well, in your showers, so that you don't add the shock of cold
water to victims. I haven't seen documentation yet to verify this, so take
it for what it is worth. It may have been a regional/state recommendation.
The link I promised is:
http://www.auburn.edu/administration/safety/standards.html
Good luck!
Judy LaDuc, HTL ASCP
>>>Jill Cox <jcox90 <@t> yahoo.com> 12/12/05 10:48 am >>>
Hello Histonetters!!
Just wondering if anyone knows about regs for an emergency shower in a
Pathology lab? I can't find anything in CAP checklist or OSHA on this. Is
there somewhere else I can look? Who all has showers? Thanks in advance,
Jill
Jill Cox HT (ASCP)
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 5
Date: Mon, 12 Dec 2005 12:56:42 -0600
From: "Poteete, Jacquie A." <japoteete <@t> saintfrancis.com>
Subject: RE: [Histonet] Emergency showers in Lab
To: 'Patsy Ruegg' <pruegg <@t> ihctech.net>, "'Weems, Joyce'"
<JWEEMS <@t> sjha.org>, 'Jill Cox' <jcox90 <@t> yahoo.com>,
"'Histonet <@t> Lists.
Utsouthwestern. Edu'" <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<D423BBB85E91D411A38D42003000996506973F67 <@t> mailnt1.saintfrancis.com>
Content-Type: text/plain
We do,also.
Jacquie Poteete MT(ASCP)QIHC
Lead Technologist, IHC Laboratory
Saint Francis Hospital, Tulsa, OK
-----Original Message-----
From: Patsy Ruegg [mailto:pruegg <@t> ihctech.net]
Sent: Monday, December 12, 2005 12:13 PM
To: 'Weems, Joyce'; 'Jill Cox'; 'Histonet <@t> Lists. Utsouthwestern. Edu'
Subject: RE: [Histonet] Emergency showers in Lab
We do.
Patsy Ruegg, HT(ASCP)QIHC
IHCtech, LLC
Fitzsimmons BioScience Park
12635 Montview Blvd. Suite 216
Aurora, CO 80010
P-720-859-4060
F-720-859-4110
wk email pruegg <@t> ihctech.net
web site www.ihctech.net
This email is confidential and intended solely for the use of the Person(s)
('the intended recipient') to whom it was addressed. Any views or opinions
presented are solely those of the author. It may contain information that is
privileged & confidential within the meaning of applicable law. Accordingly
any dissemination, distribution, copying, or other use of this message, or
any of its contents, by any person other than the intended recipient may
constitute a breach of civil or criminal law and is strictly prohibited. If
you are NOT the intended recipient please contact the sender and dispose of
this e-mail as soon as possible.
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Weems, Joyce
Sent: Monday, December 12, 2005 9:07 AM
To: Jill Cox; Histonet <@t> Lists. Utsouthwestern. Edu
Subject: RE: [Histonet] Emergency showers in Lab
We do.
Joyce Weems
Pathology Manager
Saint Joseph's Hospital of Atlanta
404-851-7376
404-851-7831 - fax
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu]On Behalf Of Jill Cox
Sent: Monday, December 12, 2005 10:49 AM
To: Histonet <@t> Lists. Utsouthwestern. Edu
Subject: [Histonet] Emergency showers in Lab
Hello Histonetters!!
Just wondering if anyone knows about regs for an emergency shower in a
Pathology lab? I can't find anything in CAP checklist or OSHA on this. Is
there somewhere else I can look? Who all has showers? Thanks in advance,
Jill
Jill Cox HT (ASCP)
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Confidentiality Notice ** The information contained in this message may be
privileged and is confidential information intended for the use of the
addressee listed above. If you are neither the intended recipient nor the
employee or agent responsible for delivering this message to the intended
recipient, you are hereby notified that any disclosure, copying,
distribution or the taking of any action in reliance on the contents of this
information is strictly prohibited. If you have received this communication
in error, please notify us immediately by replying to the message and
deleting it from your computer. Thank you. Saint Joseph's Health System,
Inc.
_______________________________________________
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------------------------------
Message: 6
Date: Mon, 12 Dec 2005 13:57:22 -0500
From: Ada Feldman <adafeldman <@t> anatechltdusa.com>
Subject: Re: [Histonet] Emergency showers in Lab
To: Jill Cox <jcox90 <@t> yahoo.com>
Cc: "Histonet <@t> Lists. Utsouthwestern. Edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <876E5143-D75B-40AB-940A-41E4CF7E0186 <@t> anatechltdusa.com>
Content-Type: text/plain; charset=US-ASCII; delsp=yes;
format=flowed
Jill,
In the Formaldehyde Standard 29 CFR 1910.1048 (i) (2) it states "If
employees may become splashed with solutions containing 1% or greater
formaldehyde, for example, because of equipment failure or improper
work practices, the employer shall provide conveniently located quick
drench showers and assure that affected employees use these facilities.
Ada T. Feldman
ANATECH LTD.
1020 Harts Lake Road
Battle Creek, MI 49015
Phone: 800.262.8324
Fax: 269.964.8084
email: adafeldman <@t> anatechltdusa.com
On Dec 12, 2005, at 10:48 AM, Jill Cox wrote:
> Hello Histonetters!!
> Just wondering if anyone knows about regs for an emergency shower
> in a Pathology lab? I can't find anything in CAP checklist or OSHA
> on this. Is there somewhere else I can look? Who all has showers?
> Thanks in advance, Jill
>
>
> Jill Cox HT (ASCP)
>
>
>
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Ada T. Feldman
ANATECH LTD.
1020 Harts Lake Road
Battle Creek, MI 49015
Phone: 800.262.8324
Fax: 269.964.8084
email: adafeldman <@t> anatechltdusa.com
website: www.anatechltdusa.com
------------------------------
Message: 7
Date: Mon, 12 Dec 2005 13:24:13 -0600
From: "Jackie M O'Connor" <Jackie.O'Connor <@t> abbott.com>
Subject: [Histonet] IHC Contractor
To: "Histonet <@t> Lists. Utsouthwestern. Edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <OFEDD9B90B.5B2CD1BD-ON862570D5.006A7945 <@t> abbott.com>
Content-Type: text/plain; charset="us-ascii"
A colleague of mine is looking for a contract lab to perform IHC with a
quick TAT - any takers?
Jackie
------------------------------
Message: 8
Date: Mon, 12 Dec 2005 13:42:55 -0800 (PST)
From: Steven Coakley <sjchtascp <@t> yahoo.com>
Subject: [Histonet] IHC on coverglass
To: Histonet <@t> lists.utsouthwestern.edu
Message-ID: <20051212214255.99767.qmail <@t> web90201.mail.scd.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1
Does anyone out there do IHC on coverglass. Appears to be a "tad" tricky.
Steve
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Message: 9
Date: Mon, 12 Dec 2005 13:46:19 -0800 (PST)
From: Loralei Dewe <lldewe <@t> ucdavis.edu>
Subject: Re: [Histonet] IHC on coverglass
To: Steven Coakley <sjchtascp <@t> yahoo.com>
Cc: Histonet <@t> lists.utsouthwestern.edu
Message-ID: <Pine.GSO.4.58.0512121346070.24063 <@t> vici.ucdavis.edu>
Content-Type: TEXT/PLAIN; charset=US-ASCII
Yep, adherent cells?
Lorie
On Mon, 12 Dec 2005, Steven Coakley wrote:
> Does anyone out there do IHC on coverglass. Appears to be a "tad" tricky.
>
> Steve
>
>
> ---------------------------------
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> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
------------------------------
Message: 10
Date: Mon, 12 Dec 2005 15:54:53 -0600
From: "Sebree Linda A." <la.sebree <@t> hosp.wisc.edu>
Subject: RE: [Histonet] IHC on coverglass
To: "Steven Coakley" <sjchtascp <@t> yahoo.com>,
<Histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<D6B654003615874B873E15BA680E2D2213C9C3CD <@t> uwhis-xchng1.hosp.wisc.edu>
Content-Type: text/plain; charset="us-ascii"
Only under extreme duress! In our case, we mounted the coverslips on a
regular slide and stained it on our automated instrument (Ventana). We
weren't very successful but I think it was because these were bone
marrow smears that sat around air-dried in a file for who knows how
long. We weren't informed that they had not been fixed in any manner
and we ended up losing most of the cells. In a best case scenario it
might work a whole lot better.
Linda A. Sebree
University of Wisconsin Hospital & Clinics
IHC/ISH Clinical & Research Laboratory
DM223-VA
600 Highland Ave.
Madison, WI 53792
(608)265-6596
FAX: (608)262-7174
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Steven
Coakley
Sent: Monday, December 12, 2005 3:43 PM
To: Histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] IHC on coverglass
Does anyone out there do IHC on coverglass. Appears to be a "tad"
tricky.
Steve
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Message: 11
Date: Mon, 12 Dec 2005 17:02:23 -0500
From: Caroline Bass <cbass <@t> bidmc.harvard.edu>
Subject: [Histonet] rat spinal cord longitudinal sections
To: "Histonet (E-mail)" <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <A93C0B94-6D2E-4995-A04C-CD5D20352152 <@t> bidmc.harvard.edu>
Content-Type: text/plain; charset=US-ASCII; delsp=yes; format=flowed
Hi Guys,
I have a rat that was injected with a virus that produces a
fluorescent protein. I would like to scan the entire spinal cord for
the signal and thought the best way to do this was to section the
spinal cord along the long axis. I don't know if I have the
terminology right on this. I think this should be longitudinal
sections. The histologists who is to section the tissue says that
this is impractical. I know that cross sections (coronal?) are more
common, but which method is better when we are simply screening for a
visible signal? I am worried that I will have hundreds of cross
sections to examine.
If there is a published example of this anywhere, even a picture on a
website I would love to see it. I have not been able to find
anything that is helpful in deciding which route to go.
Thanks for you help,
Caroline Bass
------------------------------
Message: 12
Date: Mon, 12 Dec 2005 18:20:42 -0500
From: "Monfils, Paul" <PMonfils <@t> Lifespan.org>
Subject: RE: [Histonet] rat spinal cord longitudinal sections
To: "'histonet <@t> lists.utsouthwestern.edu'"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<09C945920A6B654199F7A58A1D7D1FDE0171762F <@t> lsexch.lsmaster.lifespan.org>
Content-Type: text/plain; charset="iso-8859-1"
Sounds to me like a longitudinal section might be good for your specific
purpose. Cross sections are better for studying the morphology of the cord,
but if scanning the length of the cord is your objective, and you can
sacrifice the specific advantages of cross sections, than that's the way to
go. It is impractical to section the entire length of the uncut cord. But
the cord could be cut into three or four lengths, mounted one above the
other in a single block, and sectioned that way. It would be a fairly large
block, but in my lab we frequently section larger ones. If you do go with
cross sections, many of these could be mounted in a single block (15 should
be no great problem - 3 rows of 5 each). The average clinical lab, trying to
produce hundreds of slides a day on a deadline, may not have the time to
devote to such special projects. But it is certainly doable. My lab, a core
research facility, receives such special requests regularly.
> ----------
> From: histonet-bounces <@t> lists.utsouthwestern.edu on behalf of
> Caroline Bass
> Sent: Monday, December 12, 2005 2:02 PM
> To: Histonet (E-mail)
> Subject: [Histonet] rat spinal cord longitudinal sections
>
> Hi Guys,
>
> I have a rat that was injected with a virus that produces a
> fluorescent protein. I would like to scan the entire spinal cord for
> the signal and thought the best way to do this was to section the
> spinal cord along the long axis. I don't know if I have the
> terminology right on this. I think this should be longitudinal
> sections. The histologists who is to section the tissue says that
> this is impractical. I know that cross sections (coronal?) are more
> common, but which method is better when we are simply screening for a
> visible signal? I am worried that I will have hundreds of cross
> sections to examine.
>
> If there is a published example of this anywhere, even a picture on a
> website I would love to see it. I have not been able to find
> anything that is helpful in deciding which route to go.
>
> Thanks for you help,
>
> Caroline Bass
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
------------------------------
Message: 13
Date: Tue, 13 Dec 2005 00:39:11 +0000
From: crs grupo <grupo.crs <@t> gmail.com>
Subject: [Histonet] DAB info disposabel/neutralization/tratment
To: Histonet <@t> lists.utsouthwestern.edu
Message-ID: <a7aefe100512121639p1c7fd648w <@t> mail.gmail.com>
Content-Type: text/plain; charset=ISO-8859-1
Hello!
I'm doing a research about the DAB and his
tratment/neutralization/disposabel...
I need some information about DAB and what to do whit it after use.
If someone can give me any information, I appreciated.
------------------------------
Message: 14
Date: Tue, 13 Dec 2005 04:51:28 -0800 (PST)
From: Rene J Buesa <rjbuesa <@t> yahoo.com>
Subject: Re: [Histonet] DAB info disposabel/neutralization/tratment
To: crs grupo <grupo.crs <@t> gmail.com>, Histonet <@t> lists.utsouthwestern.edu
Message-ID: <20051213125128.98084.qmail <@t> web61216.mail.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1
Hello CRS Group:
DAB disposal constitutes an everyday need and there are several
approaches, the most common being to treat it with bleach until it
decolorizes but this practice is not recommended because the final product
of this treatment is not known.
DAB can be treat in the following way:
1-Prepare a stock solution (A) of 0.2 M potassium permanganate (=31.6 g/L)
and another stock sol. (B) of 2.0 M sulfuric acid (112mL conc./L).
2- Dilute the DAB solution up to a conc. that does not exceeds 0.9 mg/mL
3- To each 10 mL of the DAB sol. add 5 mL of sol. A + 5 mL of sol. B
4- Allow the mixture to react for at least 10 hours after which time it
will be non-mutagenic.
5- Add ascorbic acid in powder until the mixture is decolorized.
6- Add sodium bicarbonate to neutralize the solution (test with pH paper).
7- Now you can discard the solution down the drain, provided that your
local authorities give their approval,
OR you can give your DAB to a waste management company (WMC) but always
remembering that you, and not the WMC, will be responsible for the disposed
product.
In my laboratory we used to give our DAB to a WMC after we treated it with
bleach.
Hope this will help you!
Rene J.
crs grupo <grupo.crs <@t> gmail.com> wrote:
Hello!
I'm doing a research about the DAB and his
tratment/neutralization/disposabel...
I need some information about DAB and what to do whit it after use.
If someone can give me any information, I appreciated.
_______________________________________________
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Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
---------------------------------
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Find Great Deals on Holiday Gifts at Yahoo! Shopping
------------------------------
Message: 15
Date: Tue, 13 Dec 2005 10:42:46 -0500
From: Vernon Dailey <vd38 <@t> georgetown.edu>
Subject: [Histonet] anti-mouse PR primary Ab
To: Histonet <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <439EEBF6.1030900 <@t> georgetown.edu>
Content-Type: text/plain; charset="iso-8859-1"
Hi Histonet,
I need to stain FFPE mouse tissue for Progesterone Receptor. I have
tried Santa Cruz rabbit polyclonal but it is not showing the type of
staining that I would expect. The luminal epithelial cells of normal
mouse uterus are negative while the lamina propria stains positive. My
understanding is they should both be positive in normal uterus. Also, I
can only get the lamina propria staining at 1:50 in the uterus but when
I stain tumor tissue at 1:50 I get tremendous amounts of background
(tissue not necrotic and I see very nice, clean staining when using
Santa Cruz ER Ab at 1:1500) Can anyone recommend another product that
has been successful. Many thanks,
-Vernon Dailey
------------------------------
Message: 16
Date: Tue, 13 Dec 2005 11:12:49 -0500
From: "Smith, Allen" <asmith <@t> mail.barry.edu>
Subject: RE: [Histonet] Emergency showers in Lab
To: "Jill Cox" <jcox90 <@t> yahoo.com>
Cc: histonet <@t> lists.utsouthwestern.edu
Message-ID:
<5D2189E74151CC42BEC02906BA8996322B9139 <@t> exchsrv01.barrynet.barry.edu>
Content-Type: text/plain; charset="us-ascii"
Forget the regs: think about your own skin!
Sulfuric or nitric acid will do serious damage faster than you can
undress. If you handle either, you need a shower.
If you ever use an open flame, you need a shower. Showers not only
extinguish burning clothing; they also cool the skin underneath, thus
reducing the severity of the burn.
You should also have an eyewash station next to the shower. Keep the
water in the eyewash station clean by flushing it once a week. Even if you
wear goggles, eyewash stations are handy for washing the face around the
goggles after a splash. (Making colloidal iron solutions or opening a hot
container of decloaking buffer involves a spatter risk.)
My shower and eyewash are next to my fume hood, where I use my nasty
stuff.
Allen A. Smith, Ph.D.
Professor of Anatomy
Barry University School of Graduate Medical Sciences
Podiatric Medicine and Surgery
Miami Shores, Florida 33161
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Jill Cox
Sent: Monday, December 12, 2005 10:49 AM
To: Histonet <@t> Lists. Utsouthwestern. Edu
Subject: [Histonet] Emergency showers in Lab
Hello Histonetters!!
Just wondering if anyone knows about regs for an emergency shower in a
Pathology lab? I can't find anything in CAP checklist or OSHA on this. Is
there somewhere else I can look? Who all has showers? Thanks in advance,
Jill
Jill Cox HT (ASCP)
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E-mail transmission cannot be guaranteed to be secure or error-free as
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incomplete, or contain viruses.
Barry University - Miami Shores, FL (http://www.barry.edu)
------------------------------
Message: 17
Date: Tue, 13 Dec 2005 10:57:01 -0600
From: "Jackie M O'Connor" <Jackie.O'Connor <@t> abbott.com>
Subject: [Histonet] FFPE CD31 on mouse
To: "Histonet (E-mail)" <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <OFDD6C7995.7A704A62-ON862570D6.005CCEF0 <@t> abbott.com>
Content-Type: text/plain; charset="us-ascii"
I've been waiting for Santa Cruz to grow a new goat for the CD31 antibody
that USED TO work on FFPE murine tissue - but with no luck.
I've scoured the internet for two days looking for a viable replacement -
nada.
Does anyone have a method for murine CD31 in FFPE that works without the
Santa Cruz antibody? I've tried the Pharmingen anti-mouse with AR - but
it didn't work. Boo.
Jackie O'
------------------------------
Message: 18
Date: Tue, 13 Dec 2005 09:17:03 -0800
From: "Koelling, Ray" <koellinr <@t> amgen.com>
Subject: RE: [Histonet] FFPE CD31 on mouse
To: "'Jackie M O'Connor'" <Jackie.O'Connor <@t> abbott.com>, "Histonet
(E-mail)" <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<16834C6DFFA6004C88DE4507FB8AE544018CCA28 <@t> wa-mb4-sea.amgen.com>
Content-Type: text/plain; charset="iso-8859-1"
Jackie,
Not too long ago I posted on Histonet our CD31 procedure we've used for a
couple years. On mouse tissue using the Pharmingen antibody. Works
beautifully but need to enzyme retrieve and amplify signal. Should be in
archive.
Ray
Raymond Koelling
Pathology Research scientist
Amgen Corp.
Seattle, WA
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu]On Behalf Of Jackie M
O'Connor
Sent: Tuesday, December 13, 2005 8:57 AM
To: Histonet (E-mail)
Subject: [Histonet] FFPE CD31 on mouse
I've been waiting for Santa Cruz to grow a new goat for the CD31 antibody
that USED TO work on FFPE murine tissue - but with no luck.
I've scoured the internet for two days looking for a viable replacement -
nada.
Does anyone have a method for murine CD31 in FFPE that works without the
Santa Cruz antibody? I've tried the Pharmingen anti-mouse with AR - but
it didn't work. Boo.
Jackie O'
_______________________________________________
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http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 19
Date: Tue, 13 Dec 2005 10:30:19 -0700
From: Gayle Callis <gcallis <@t> montana.edu>
Subject: Protocols that have worked using BD PharminGen monoclonal Re:
[Histonet] FFPE CD31 on mouse
To: "Jackie M O'Connor" <Jackie.O'Connor <@t> abbott.com>,
Histonet <@t> lists.utsouthwestern.edu
Message-ID:
<6.0.0.22.1.20051213102205.01b288c8 <@t> gemini.msu.montana.edu>
Content-Type: text/plain; charset="us-ascii"; format=flowed
Jackie,
Here are two messages copied from Histonet Archives using CD31 BD Pharm MEC
13.3, on FFPE murine tissues with success:
1. I have used a rat anti mouse CD31 from Pharmingen(BD Biosciences).Cat.
#553370. I use a rabbit anti rat(mouse adsorbed)secondary-biotinylated,from
Vector Labs. For retreival, use pepsin at 37C for 20-40 min. depending on
fixation. I used the ABC detection from Vector with DAB.
2. If you are using the DAKO CD31 antibody - it will not react with mouse
endothelium. Pharmigen CD31 (MEC13.3) works well on FFPE with Vectastain
Elite-
ABC followed by NEN TSA-kit. Both work well on FFPE mouse tissues.
Barb Wright
Jamie Erickson supplied this info to me using BD Pharmingen clone, MEC
13.3, 1.25 ug/ml concentration, using proteinase K digestion on FFPE tissue.
There may be more messages in Histonet Archives for this antibody
At 09:57 AM 12/13/2005, you wrote:
>I've been waiting for Santa Cruz to grow a new goat for the CD31 antibody
>that USED TO work on FFPE murine tissue - but with no luck.
>I've scoured the internet for two days looking for a viable replacement -
>nada.
>Does anyone have a method for murine CD31 in FFPE that works without the
>Santa Cruz antibody? I've tried the Pharmingen anti-mouse with AR - but
>it didn't work. Boo.
>
>Jackie O'
>_______________________________________________
>Histonet mailing list
>Histonet <@t> lists.utsouthwestern.edu
>http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Gayle Callis
Research Histopathology Supervisor
Veterinary Molecular Biology
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610
406 994-6367
406 994-4303 (FAX)
------------------------------
Message: 20
Date: Tue, 13 Dec 2005 12:30:38 -0500
From: DDDeltour <@t> mar.med.navy.mil
Subject: [Histonet] Florida License
To: histonet <@t> lists.utsouthwestern.edu
Message-ID:
<3F500F8B416C554EBB21FF16642F72E959CD8C <@t> marxchg03.mar.med.navy.mil>
Content-Type: text/plain
Hello All,
I am looking to get my Florida License but I am currently living in
Virginia. Is there an online course that covers the required HIV class? Can
anyone give me any advice how to get this process done without much pain :-)
Thanks
Douglas D. Deltour HT(ASCP)
Histology Technician
NMCP Portsmouth Va
(757) 953-1525/1523
------------------------------
Message: 21
Date: Tue, 13 Dec 2005 10:34:11 -0700
From: Gayle Callis <gcallis <@t> montana.edu>
Subject: [Histonet] Another Histonet Archive CD31 for murine FFPE
tissue
To: Histonet <@t> lists.utsouthwestern.edu
Message-ID:
<6.0.0.22.1.20051213103225.01b07c10 <@t> gemini.msu.montana.edu>
Content-Type: text/plain; charset="us-ascii"; format=flowed
It seems most people use enzyme digestion with FFPE CD31 on mouse, rather
than an antigen retrieval i.e. with buffers/HIER.
Other message:
I just completed a project usind CD31 on FFPE mouse tissue with good
results.
The supplier of the antibody is Research Diagnostics (973-584-7093;
www.researchd.com). I used rat anti-mouse CD31, monoclonal; Catalog#
RDI-mCD31abrt. They also suggested zinc fixation, but it worked on formalin
fixation. I used DAKO's Prot K to open up the sights.
Bob Meyer
Pathology Core Facility
Northwestern University
Gayle Callis HTL, HT, MT(ASCP)
Research Histopathology Supervisor
Veterinary Molecular Biology
Montana State University
Bozeman MT 59717
------------------------------
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End of Histonet Digest, Vol 25, Issue 16
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