[Histonet] FFPE CD31 on mouse

[Angela McNabola]

Gayle is right....it is a monclonal....I was thinking of something else...thank
you!


                                                                                                                           
                      Gayle Callis                                                                                         
                      <gcallis <@t> montana.        To:       Angela McNabola <angela.mcnabola.b <@t> bayer.com>                     
                      edu>                     cc:                                                                         
                                               Subject:  Re: [Histonet] FFPE CD31 on mouse                                 
                      12/13/2005 01:27                                                                                     
                      PM                                                                                                   
                                                                                                                           




Isn't the ABCAM rat antiMouse CD34 a monoclonal, the only CD34 they have
that is polyclonal is chicken anti CD34 - that cross reacts to human, mouse
and rat?


At 11:12 AM 12/13/2005, you wrote:
>We received the new lot of CD31 from Santa Cruz, and it is working, though we
>are going to have to use it at a more concentrated diution than our current
>(1:750).
>
>We haven't had the time to see what the new concentration will be, but I'm
>guessing somewhere around 1:200 or so.  If the CD31 you have is not working,
>Santa Cruz will replace it to try it for yourself.  We got a couple of
>vials for
>free as replacements.
>
>Another alternative is CD34 (can give you some of the same information,
>depedning on your work).  Abcam has a great rat polyclonal that works well in
>FFPE mouse tissues.
>
>
>Angela
>
>
>Angela McNabola, MS, HT(ASCP)SLS, QIHC
>Bayer Helathcare
>400 Morgan Lane
>West Haven, CT 06516
>203-812-5001
>angela.mcnabola.b <@t> bayer.com
>
>
>
>
>
>                       "Jackie M
> O'Connor"
>
>                       <Jackie.O'Connor <@t> abbott.com>          To:
> "Histonet (E-mail)" <histonet <@t> lists.utsouthwestern.edu>
>                       Sent
> by:                              cc:
>
>                       histonet-bounces <@t> lists.utsouth        Subject:
> [Histonet] FFPE CD31 on mouse
>                       western.edu
>
>
>
>
>
>                       12/13/2005 11:57
> AM
>
>
>
>
>
>
>
>I've been waiting for Santa Cruz to grow a new goat for the CD31 antibody
>that USED TO work on FFPE murine tissue - but with no luck.
>I've scoured the internet for two days looking for a viable replacement -
>nada.
>Does anyone have a method for murine CD31 in FFPE that works without the
>Santa Cruz antibody?  I've tried the Pharmingen anti-mouse with AR - but
>it didn't work.  Boo.
>
>Jackie O'
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Gayle Callis
Research Histopathology Supervisor
Veterinary Molecular Biology
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610
406 994-6367
406 994-4303 (FAX)