Protocols that have worked using BD PharminGen monoclonal Re:
[Histonet] FFPE CD31 on mouse
gcallis <@t> montana.edu
Tue Dec 13 11:30:19 CST 2005
Here are two messages copied from Histonet Archives using CD31 BD Pharm MEC
13.3, on FFPE murine tissues with success:
1. I have used a rat anti mouse CD31 from Pharmingen(BD Biosciences).Cat.
#553370. I use a rabbit anti rat(mouse adsorbed)secondary-biotinylated,from
Vector Labs. For retreival, use pepsin at 37C for 20-40 min. depending on
fixation. I used the ABC detection from Vector with DAB.
2. If you are using the DAKO CD31 antibody - it will not react with mouse
endothelium. Pharmigen CD31 (MEC13.3) works well on FFPE with Vectastain
ABC followed by NEN TSA-kit. Both work well on FFPE mouse tissues.
Jamie Erickson supplied this info to me using BD Pharmingen clone, MEC
13.3, 1.25 ug/ml concentration, using proteinase K digestion on FFPE tissue.
There may be more messages in Histonet Archives for this antibody
At 09:57 AM 12/13/2005, you wrote:
>I've been waiting for Santa Cruz to grow a new goat for the CD31 antibody
>that USED TO work on FFPE murine tissue - but with no luck.
>I've scoured the internet for two days looking for a viable replacement -
>Does anyone have a method for murine CD31 in FFPE that works without the
>Santa Cruz antibody? I've tried the Pharmingen anti-mouse with AR - but
>it didn't work. Boo.
>Histonet mailing list
>Histonet <@t> lists.utsouthwestern.edu
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