[Histonet] IHC on coverglass

[Sebree Linda A.]

Only under extreme duress!  In our case, we mounted the coverslips on a
regular slide and stained it on our automated instrument (Ventana).  We
weren't very successful  but I think it was because these were bone
marrow smears that sat around air-dried in a file for who knows how
long.  We weren't informed that they had not been fixed in any manner
and we ended up losing most of the cells.  In a best case scenario it
might work a whole lot better.

Linda A. Sebree
University of Wisconsin Hospital & Clinics
IHC/ISH Clinical & Research Laboratory
DM223-VA
600 Highland Ave.
Madison, WI 53792
(608)265-6596
FAX:  (608)262-7174



-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Steven
Coakley
Sent: Monday, December 12, 2005 3:43 PM
To: Histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] IHC on coverglass


Does anyone out there do IHC on coverglass.  Appears to be a "tad"
tricky.
   
  Steve

			
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