[Histonet] (no subject)

[Rogerson Kemlo (ELHT) Pathology]

But it then would not be 'crispy'. I have suggested poor fixation which
would mean you are relying on the fixative properties of ethanol; which
causes tissue to go hard. Rapidly processed tissue usually result in
poor processing which is 'soft' not 'hard', IMHO.

Good preliminary fixative will prevent a lot of problems subsequently.

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Crick
Tony (Morecambe Bay Hospitals NHS Trust)
Sent: 12 December 2005 14:52
To: 'Patterson, Pat'; 'histonet <@t> lists.utsouthwestern.edu'
Subject: RE: [Histonet] (no subject)

It sounds like processing issue being carried out too fast.
Tony

-----Original Message-----
From: Patterson, Pat [mailto:PatPatterson <@t> mhd.com] 
Sent: 12 December 2005 14:48
To: 'histonet <@t> lists.utsouthwestern.edu'
Subject: [Histonet] (no subject)


Hello to all HistoNet folks.  

 

Our lab is having inconsistent occurrences of "crispy" biopsies -
especially
Livers. They feel crispy during embedding and sectioning and the
sections
appear to have cracking - separating the tissue in unnatural directions.

 

These are fixed in Carson Millonig formalin and then processed with 10%
NBF,
70% alc, 95% alc, Abs alc, xylene to paraffin - total process about 2.5
hours.  They are held in the 10% NBF at the beginning of the processing
schedule and there is no heat on the retort until the paraffin steps.

 

The biopsies are placed in "micro-cassettes" with no wrapping (these
cassettes are not the micro-screen cassettes ones with metal like
screening).

 

We'd appreciate any ideas you might have - especially since our liver
transplant service is increasing in volume as I type!

 

Thanks!

 

Pat Patterson

Manager, AP

Methodist Dallas Medical Center

(214) 947-3538

 

 



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