[Histonet] (no subject)

[Crick Tony]

It sounds like processing issue being carried out too fast.
Tony

-----Original Message-----
From: Patterson, Pat [mailto:PatPatterson <@t> mhd.com] 
Sent: 12 December 2005 14:48
To: 'histonet <@t> lists.utsouthwestern.edu'
Subject: [Histonet] (no subject)


Hello to all HistoNet folks.  

 

Our lab is having inconsistent occurrences of "crispy" biopsies - especially
Livers. They feel crispy during embedding and sectioning and the sections
appear to have cracking - separating the tissue in unnatural directions.

 

These are fixed in Carson Millonig formalin and then processed with 10% NBF,
70% alc, 95% alc, Abs alc, xylene to paraffin - total process about 2.5
hours.  They are held in the 10% NBF at the beginning of the processing
schedule and there is no heat on the retort until the paraffin steps.

 

The biopsies are placed in "micro-cassettes" with no wrapping (these
cassettes are not the micro-screen cassettes ones with metal like
screening).

 

We'd appreciate any ideas you might have - especially since our liver
transplant service is increasing in volume as I type!

 

Thanks!

 

Pat Patterson

Manager, AP

Methodist Dallas Medical Center

(214) 947-3538

 

 



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