[Histonet] (no subject)

Crick Tony Tony.Crick <@t> rli.mbht.nhs.uk
Mon Dec 12 08:51:35 CST 2005

It sounds like processing issue being carried out too fast.

-----Original Message-----
From: Patterson, Pat [mailto:PatPatterson <@t> mhd.com] 
Sent: 12 December 2005 14:48
To: 'histonet <@t> lists.utsouthwestern.edu'
Subject: [Histonet] (no subject)

Hello to all HistoNet folks.  


Our lab is having inconsistent occurrences of "crispy" biopsies - especially
Livers. They feel crispy during embedding and sectioning and the sections
appear to have cracking - separating the tissue in unnatural directions.


These are fixed in Carson Millonig formalin and then processed with 10% NBF,
70% alc, 95% alc, Abs alc, xylene to paraffin - total process about 2.5
hours.  They are held in the 10% NBF at the beginning of the processing
schedule and there is no heat on the retort until the paraffin steps.


The biopsies are placed in "micro-cassettes" with no wrapping (these
cassettes are not the micro-screen cassettes ones with metal like


We'd appreciate any ideas you might have - especially since our liver
transplant service is increasing in volume as I type!




Pat Patterson

Manager, AP

Methodist Dallas Medical Center

(214) 947-3538




This electronic transmission contains information from Methodist Health 
System and should be considered confidential and privileged.  The 
information contained in the above messages is intended only for the 
use of the individual(s) and entity(ies) named above.  If you are not 
the intended recipient, be aware that any disclosure, copying, 
distribution, or use of this information is prohibited. If you receive 
this transmission in error, please notify the sender immediately by 
return e-mail.  Methodist Health System, its subsidiaries and 
affiliates hereby claim all applicable privileges related to the 
transmission of this communication.
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu

DISCLAIMER: This e-mail is confidential and privileged. If you are not the
intended recipient please accept our apologies; please do not disclose, copy
or distribute information in this e-mail or take any action in reliance on
its contents: to do so is strictly prohibited and may be unlawful. Please
inform postmaster <@t> rli.mbht.nhs.uk that this message has gone astray before
deleting it.  Comments or opinions expressed in this email are those of
their respective contributors only. The views expressed do not represent the
views of the Trust, its management or employees. Morecambe Bay Hospitals NHS
Trust is not responsible and disclaims any and all liability for the content
of comments written within.Thank you for your co-operation.

More information about the Histonet mailing list