[Histonet] Tap Water in Staining

Walters, Katherine S katherine-walters <@t> uiowa.edu
Thu Dec 8 10:52:24 CST 2005

Is that why they call it Scott's tap water?

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Ian
Sent: Thursday, December 08, 2005 10:35 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: Fwd: Re: [Histonet] Tap Water in Staining

         Benefit of living in Glasgow, the tap water. You can drink 
it. bathe in it, make whisky with it and blue sections.

>Distilled or deionized ("pure") water nearly always has a pH
>between 5 and 6 because of dissolved carbon dioxide (carbonic
>acid) from the air. To change the colour of haemalum-stained
>nuclei from red to blue the pH should be 7 or higher. Hard tap
>water is neutral or slightly alkaline, and the dissolved calcium
>bicarbonate can serve as a buffer. A drop of anything alkaline
>will bring the pH of pure water
>to a little over 7. I use limewater (saturated, that's about
>0.3%, aqueous calcium hydroxide) because Ca(OH)2 is harmless and
>John Kiernan
>Anatomy, UWO
>London, Canada
>"Parker, Helayne" wrote:
> >
> > In using tap water for an H & E stain is there any documentation or
> > testing that needs to be done to confirm that the tap water is ok
> > use.  They have been using DI water here and there were complaints
> > slides being too pink from the Pathologist.  I put the tap water
> > the regimen instead as I have always used tap for H & E staining
> > is now fine - no problems.  But I was asked by the other tech is
> > was acceptable.  Having never been a supervisor I am not aware of
> > documentation needed. Can anyone advise.
> >
> > Helayne Parker, HT (A.S.C.P.)
> > Histology Section Head
> > Skaggs Community Health Center
> > Branson, MO
> >
> > _______________________________________________
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> > Histonet <@t> lists.utsouthwestern.edu
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Dr. Ian Montgomery,
IBLS Support Services,
Graham Kerr Building,
Institute of Biomedical & Life Sciences,
University of Glasgow,
G12 8QQ.
Tel: 0141 339 8855
Office: 4652
Lab: 6644.
Pager: 07623 975451
e-mail: ian.montgomery <@t> bio.gla.ac.uk  

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