Fwd: Re: [Histonet] Tap Water in Staining

[Ian Montgomery]

         Benefit of living in Glasgow, the tap water. You can drink 
it. bathe in it, make whisky with it and blue sections.



>Distilled or deionized ("pure") water nearly always has a pH
>between 5 and 6 because of dissolved carbon dioxide (carbonic
>acid) from the air. To change the colour of haemalum-stained
>nuclei from red to blue the pH should be 7 or higher. Hard tap
>water is neutral or slightly alkaline, and the dissolved calcium
>bicarbonate can serve as a buffer. A drop of anything alkaline
>will bring the pH of pure water
>to a little over 7. I use limewater (saturated, that's about
>0.3%, aqueous calcium hydroxide) because Ca(OH)2 is harmless and
>cheap.
>
>John Kiernan
>Anatomy, UWO
>London, Canada
>------------------------------------
>"Parker, Helayne" wrote:
> >
> > In using tap water for an H & E stain is there any documentation or
> > testing that needs to be done to confirm that the tap water is ok for
> > use.  They have been using DI water here and there were complaints of
> > slides being too pink from the Pathologist.  I put the tap water into
> > the regimen instead as I have always used tap for H & E staining -color
> > is now fine - no problems.  But I was asked by the other tech is that
> > was acceptable.  Having never been a supervisor I am not aware of any
> > documentation needed. Can anyone advise.
> >
> > Helayne Parker, HT (A.S.C.P.)
> > Histology Section Head
> > Skaggs Community Health Center
> > Branson, MO
> >
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>
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Dr. Ian Montgomery,
Histotechnology,
IBLS Support Services,
Graham Kerr Building,
Institute of Biomedical & Life Sciences,
University of Glasgow,
Glasgow,
G12 8QQ.
Tel: 0141 339 8855
Office: 4652
Lab: 6644.
Pager: 07623 975451
e-mail: ian.montgomery <@t> bio.gla.ac.uk