[Histonet] Brdu staining fixed cultured cells.

[Steven Coakley]

Good afternoon everyone,
   
  I'm working witha scientist who is attempting to stain previously fixed cultured cells in "wells" and cover slips with anti-Brdu.  He has fixed the cells 1st in methanol, air dryed, then in 10%formalin/PBS and again air dryed.  Our anti-BrdU/DNAase works great on tissue but not on the fixed/air dryed cultured cells.  We tried a 30 minute 0.1% Saponin step to help permeablize the cells but w/o success.  Any ideas.
   
  Steve

			
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