[Histonet] Anti-DIG peroxidase problem

[Mikael Niku]

Dear Histonetters,

I'm trying to set up tyramide amplification for my in situ 
hybridizations with digoxigenin probes.
Unfortunately it seems as if the anti-DIG-POD by Roche doesn't work as 
well as the anti-DIG-AP I'm normally using.
Basically, I get a weaker signal with anti-DIG-POD + tyramide 
amplification than with unamplified anti-DIG-AP.

I have also tried biotinylated probes to avoid using anti-DIG-POD 
altogether, but so far I haven't got any signal at all with them.

Any ideas, anyone?

With best regards,
Mikael Niku

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  Mikael Niku             URL: www.helsinki.fi/~mniku/
  University of Helsinki  Dept. Basic Veterinary Sciences

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