[Histonet] specific Ig detection

Gayle Callis gcallis <@t> montana.edu
Mon Dec 5 16:02:18 CST 2005

You say "I know that it produces anaphlylaxis and will produce IgE and IgA 
so isn't this called the Host response.  In other words the host infected 
by the "bug" responds to this by producing IgE (an allergic response as is 
anaphylaxis  along with IgA)  I don't think the bug is producing these Ig's.

I suggest you try to get your "bug" identified via some microbiological 
means, such as a diagnostic culturing protocol.

The serum of an animal infected with this infectious agent should contain 
antiserum to the agent, hence antiserum i.e. antibodies to the bug should 
be present in the serum of the infected host.   So if you infected mouse 
cells with the bug, then the serum of the mouse will contain mouse antiBug 
antibodies - not necessarily IgA.

I guess I am confused on what you are trying to do here.

  At 02:27 PM 12/5/2005, you wrote:
>I am a student taking an immunobiology course.  I was wondering if someone 
>could help point me in the right direction.  I have an unknown infections 
>If I isolate the IgE's from the serum I will get all IgE's even those not 
>specific to the bug.  How can I seperate the two and get the specific 
>antigen?  Can I use a protein which would be specific to the bug but how 
>do I know what protein?
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>Histonet <@t> lists.utsouthwestern.edu

Gayle Callis
Research Histopathology Supervisor
Veterinary Molecular Biology
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610
406 994-6367
406 994-4303 (FAX)

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