[Histonet] Extracellular matrix proteins fixation.

[Diego J. Rodríguez Gil]

Hi everybody:

   I am brand new on the list, so I do not know if this question was posted
before. I apologize for this.
   I am trying to characterize a family of secreted proteins in the Nervous
System by immunohistochemistry, and with the regular fixation protocol that we
used (intracardiac perfusion with 4% PFA), I am not getting any good results.
Not even after some antigen-retrieval procedures. Before trying random things,
does anybody have a good protocol to get extracellular proteins well fixed? It
doesn't matter if it is set up for some other tissue, I would be glad to try
other protocol(s) if there are any available.
   Thank you very much for your help,
   Best regards,
   Diego.

-- 
Diego J. Rodríguez Gil
Postdoctoral Associate
Department of Neurosurgery
Yale University School of Medicine
PO Box 208082
333 Cedar St, FMB-430
New Haven CT, 06520-8082
U.S.A.
E-mail: diego.rodriguezgil <@t> yale.edu