[Histonet] Paraffin sections

[Colleen Forster]

I personally don't like to do that at all. I think you loose staining 
after they have sat, doesn't matter if it is in dh2o,buffer or sir 
dried(ouch!). Maybe others have had success. You would be better off 
leaving them until the next day.

Colleen Forster
U of MN

Molinari, Betsy wrote:

>Hi,
>
>  I have heard that paraffin sections that have been deparaffinized and
>run down to DH20 but for some reason cannot be stained that day, can be
>left to air dry and then be placed back into H2O when they are ready for
>staining. Does anyone have any experience with this technique? I usually
>just leave them in DH2O till I can get to them, usually the next day.
>
>Thanks.
>
> 
>
>Betsy Molinari HT (ASCP)
>
>Texas Heart Institute
>
>Cardiovascular Pathology
>
>6770 Bertner Ave.
>
>Houston,TX 77030
>
>832-355-6524
>
>832-355-6812 (fax)
>
> 
>
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